国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (6): 674-676.

• 论著 • 上一篇    下一篇

山奈酚对BaP引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响

连立芬，陈亚琼，侯海燕，曹波，吴念   

  1. 050000 石家庄，河北医科大学（连立芬）；天津市武警医学院附属医院妇产科（连立芬，陈亚琼，侯海燕，吴念）；天津武警后勤学院（曹波）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 陈亚琼

Effect of Kaempferol on the BaP Induced Apoptosis in Human Chorionic Trophoblast Cells HTR8/SV neo

LIAN Li-fen，CHEN Ya-qiong，HOU Hai-yan，CAO Bo，WU Nian   

  1. Hebei Medical University，Shijiazhuang 050000，China（LIAN Li-fen）；Department of Obstetrics and Gynecology，Affiliated Hospital of the Chinese People′s Armed Forces Medical School，Tianjin 300162，China（LIAN Li-fen，CHEN Ya-qiong，HOU Hai-yan，WU Nian）；The Chinese People′s Armed Forces Medical School，Tianjin 300162，China（CAO Bo）
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-12-15 Online:2014-12-15

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被引次数

3

摘要： 目的：研究山奈酚对苯并芘（BaP）引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响。方法：通过四甲基偶氮唑盐（MTT）法观察及测定，最终选取10 μmol/L BaP和0.01，0.1，1 μmol/L山奈酚共同处理人HTR8/SV neo细胞48 h，光镜下观察不同浓度BaP作用下细胞生长情况，吉姆萨（Gimesa）染色及Hochest33342荧光染色观察细胞凋亡情况，利用流式细胞术测定细胞凋亡率变化。结果：①流式细胞术测定结果表明BaP单独作用细胞存活率为（72.58±0.29）%，与0.01，0.1，1 μmol/L山奈酚同时作用时细胞存活率分别为（84.96±1.34）%、（89.54±1.64）%和（91.01±1.26）%，两者同时作用组细胞存活率均高于BaP单独作用组，差异均有统计学意义（P＜0.01）。②单独BaP作用可引起细胞染色质浓缩，出现凋亡小体等典型凋亡形态、细胞核致密浓染、碎裂状。结论：山奈酚对BaP引起的HTR8/SV neo细胞凋亡有明显的抑制作用。

关键词: 空气污染, 滋养层, 胎盘, 凋亡, DNA损伤

Abstract: Objective：To study the effect of kaempferol on the BaP induced apoptosis in human chorionic trophoblast cells HTR8/SV neo. Methods：10 μmol/L BaP were added 0.01，0.1，1 μmol/L kaempferol jointly deal with human HTR8/SV neo cells 48 h，observed cells growth under the inverted microscope，Gimesa staining and Hochest33342 fluorescent staining observed morphologic changes，determination of apoptosis rate changes by flow cytometry. Results：① Flow cytometry results showed that BaP alone cell viability was （72.58±0.29）%, with 0.01,0.1,1 μmol/L kaempferol the role of cell viability was （84.96±1.34）%,（89.54±1.64）% and （91.01±1.26）%, respectively, compared with BaP alone group, the differences were statistically significant（P＜0.01）. ②BaP alone can cause cell chromatin condensation，apoptotic bodies and other typical apoptotic morphology，dense hyperchromatic nuclei，fragmentation shape. Conclusions：Kaempferol showed a protective effect on BaP induced apoptosis in HTR8/SV neo cells.

Key words: Air pollution, Trophoblasts, Placenta, Apoptosis, DNA damage