国际妇产科学杂志

国际妇产科学杂志 ›› 2019, Vol. 46 ›› Issue (4): 426-429.

• 论著 • 上一篇    下一篇

miR-18a对人正常滋养细胞中MMP-2、MMP-9表达的影响

杨洋，陈蕊，王稳莹，刘成娟   

  1. 710077 西安医学院第一附属医院妇产科（杨洋，陈蕊，王稳莹）；中国人民解放军空军军医大学唐都医院妇产科（刘成娟）
  • 收稿日期:2018-11-12 修回日期:2019-03-11 出版日期:2019-08-15 发布日期:2019-08-15
  • 通讯作者: 刘成娟，E-mail：liuchengjuan@sina.com E-mail:123632654@qq.com
  • 基金资助:
    陕西省自然科学基础研究计划项目（2018JM7115）；陕西省教育厅专项科研计划项目（18JK0676）

Effect of MiR-18a on the Expression of MMP-2 and MMP-9 in Human Normal Trophoblast Cells

YANG Yang，CHEN Rui，WANG Wen-ying，LIU Cheng-juan   

  1. Department of Obstetrics and Gynecology，The First Affiliated Hospital of Xi′an Medical University，Xi′an 710077，China（YANG Yang，CHEN Rui，WANG Wen-ying）；Department of Obstetrics and Gynecology，Tangdu Hospital，Chinese People′s Liberation Army Air Force Military Medical University，Xi′an 710038，China（LIU Cheng-juan）
  • Received:2018-11-12 Revised:2019-03-11 Published:2019-08-15 Online:2019-08-15
  • Contact: LIU Cheng-juan，E-mail：liuchengjuan@sina.com E-mail:123632654@qq.com

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摘要： 目的：研究子痫前期（pre-eclapmpsia，PE）相关微小RNA-18a（miR-18a）对人正常滋养细胞（HTR-8细胞）中基质金属蛋白酶2（MMP-2）、MMP-9的影响。方法：按照HTR-8细胞中miR-18a的表达情况分为3组（每组重复5次）：miR-18a过表达组、miR-18a抑制组、阴性对照组；实时定量聚合酶链反应（RT-qPCR）检测转染后HTR-8细胞中miR-18a mRNA的表达量；RT-qPCR和蛋白质印迹（Western blotting）检测转染后HTR-8细胞中MMP-2、MMP-9 mRNA和蛋白表达量。结果：miR-18a过表达组miR-18a mRNA表达量比阴性对照组升高，miR-18a抑制组miR-18a mRNA表达量比阴性对照组降低，差异均有统计学意义（均P＜0.001）。miR-18a抑制组MMP-2、MMP-9 mRNA和蛋白表达量比阴性对照组降低，差异均有统计学意义（均P＜0.001）。结论：miR-18a影响人正常滋养细胞中MMP-2、MMP-9在mRNA及蛋白水平的表达，可能通过该途径参与对滋养细胞浸润能力的调控。

关键词: 微RNAs, 先兆子痫, 基质金属蛋白酶2, 基质金属蛋白酶9, 滋养细胞

Abstract: Objective：To investigate the effect of pre-eclapmsia related miR-18a on the expression of matrix metallopeptidase 2 (MMP-2) and MMP-9 in human normal trophoblast cells (HTR-8). Methods：According to the expression of miR-18a in HTR-8 cells, it was divided into 3 groups (5 times in each group): miR-18a overexpression group, miR-18a inhibition group, negative control group. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-18a mRNA in HTR-8 cells after transfection. The mRNA and protein levels of MMP-2 and MMP-9 in HTR-8 cells were detected by RT-qPCR and Western blotting after transfection. Results：The expression of miR-18a mRNA in miR-18a overexpression group was higher than that in negative control group (P＜0.001). The expression of miR-18a mRNA in miR-18a inhibition group was lower than that in negative control group (P＜0.001). The expression of MMP-2 and MMP-9 mRNA and protein in the miR-18a inhibition group was lower than that in the negative control group, and the difference was statistically significant (both P＜0.001). Conclusions：MiR-18a could affect the expression of MMP-2 and MMP-9 in human normal trophoblast cells at mRNA and protein levels, and may be involved in the regulation of trophoblast infiltration through this pathway.

Key words: MicroRNAs, Pre-eclampsia, Matrix metalloproteinase 2, Matrix metalloproteinase 9, Trophoblastic cell