国际妇产科学杂志

国际妇产科学杂志 ›› 2019, Vol. 46 ›› Issue (4): 422-425.

• 论著 • 上一篇    下一篇

miR-210和HIF-1α在子痫前期患者胎盘中的表达关系研究

陈敏，王明珠，刘增佑   

  1. 518000 广东省深圳市南山区人民医院产科
  • 收稿日期:2019-03-20 修回日期:2019-04-03 出版日期:2019-08-15 发布日期:2019-08-15
  • 通讯作者: 陈敏，E-mail：chenmin19863@163.com E-mail:cmyy86@sina.com
  • 基金资助:
    深圳市南山区科技计划项目（2018038）

Study on Expression Relationship of MiR-210 and HIF-1α in Placenta of Patients with Pre-Eclampsia

CHEN Min，WANG Ming-zhu，LIU Zeng-you   

  1. People′s Hospital of Nanshan District，Shenzhen 518000，Guangdong Province，China
  • Received:2019-03-20 Revised:2019-04-03 Published:2019-08-15 Online:2019-08-15
  • Contact: CHEN Min，E-mail：chenmin19863@163.com E-mail:cmyy86@sina.com

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摘要： 目的：检测子痫前期（PE）患者胎盘中miR-210和缺氧诱导因子1α（HIF-1α）表达水平，分析二者表达相关性及其与PE患者临床病理的关系。方法：选取深圳市南山区人民医院（我院）2016年1月—2018年2月收治的46例PE孕妇为研究对象，根据子痫前期病情程度分为轻度组21例和重度组25例，另选择同期在我院孕检正常并在妊娠结束行剖宫产孕妇34例为对照组，通过常规指标检测分析PE患者临床症状，采用荧光定量聚合酶链反应（PCR）检测胎盘中miR-210、HIF-1α mRNA表达，采用免疫组织化学染色法检测胎盘中HIF-1α表达，分析PE患者胎盘中miR-210和HIF-1α的表达相关性及与患者基本病理资料的关系。受试者工作特征（ROC）曲线分析miR-210、HIF-1α mRNA表达水平对PE的诊断价值。结果：PE患者收缩压（SBP）、舒张压（DBP）和尿蛋白（PRO）水平高于对照组（P＜0.05），且重度PE组高于轻度PE组（P＜0.05）。PE患者胎盘组织中miR-210、HIF-1α水平高于对照组（P＜0.05），且重度PE组高于轻度PE组（P＜0.05）。胎盘组织中miR-210、HIF-1α水平与PE患者SBP、DBP、PRO水平及PE严重程度相关（P＜0.05）。PE患者胎盘中miR-210与HIF-1α表达呈正相关（P＜0.05）。ROC分析显示miR-210、HIF-1α mRNA的截断值分别为1.06、1.27时，诊断PE时的敏感度分别为80.43%和78.26%，特异度分别为70.59%和82.35%。结论：miR-210和HIF-1α在PE患者胎盘组织中高表达，二者表达水平呈正相关，共同参与PE发生、发展。

关键词: 先兆子痫, 微RNAs, 缺氧诱导因子1, α亚基, 胎盘

Abstract: Objective: To detect the expression of micro RNA-210 (miR-210) and hypoxia-inducible factor 1α (HIF-1α) in placenta of pre-eclampsia (PE) patients, and analyze the relationship between them as well as the correlation between the expression and clinicopathological characteristics of PE patients. Methods: 46 pregnant women with PE admitted to our hospital from January 2016 to February 2018 were selected as the study subjects. According to the severity of PE, 21 cases were divided into mild group and 25 cases into severe group. Another 34 pregnant women with normal pregnancy examination and cesarean delivery were selected as the control group. The clinical symptoms of PE patients were analyzed by routine biochemical indicators test. Fluorescent quantitative PCR was used to detect the expression of miR-210 and HIF-1α mRNA in placenta. Immunohistochemical staining was used to detect the expression of HIF-1α in placenta. And the correlation between the expressions of miR-210 and HIF-1α in placenta of PE patients and their relationships with the basic pathological data of patients were analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of miR-210 and HIF-1α mRNA expression levels in PE. Results: Systolic blood pressure (SBP), diastolic blood pressure (DBP) and urinary protein (PRO) levels in PE patients were significantly higher than those in the control group (P＜0.05), and severe PE group were significantly higher than mild PE group (P＜0.05). The levels of miR-210 and HIF-1α in placenta of PE patients were significantly higher than those of control group (P＜0.05), and severe PE group were significantly higher than mild PE group (P＜0.05). The levels of miR-210 and HIF-1α in placenta were correlated with the levels of SBP, DBP, PRO and the severity of PE (P＜0.05). The expressions of miR-210 and HIF-1α in placenta of PE patients were positively correlated (P＜0.05). ROC analysis showed that the cut-off values of miR-210 and HIF-1α mRNA were 1.06 and 1.27, and the sensitivity of PE diagnosis was 80.43% and 78.26%, the specificity was 70.59% and 82.35%. Conclusions: MiR-210 and HIF-1α are highly expressed in the placenta of PE patients, and they are positively correlated with each other, and participate in the occurrence and development of PE disease.

Key words: Pre-eclampsia, MicroRNAs, Hypoxia-inducible factor 1, alpha subunit, Placenta