多囊卵巢综合征鼠类动物模型的构建

doi:10.12280/gjfckx.20210970

摘要/Abstract

摘要：

多囊卵巢综合征（polycystic ovarian syndrome,PCOS）是育龄期女性常见的一种内分泌代谢性疾病,病因复杂,发病率高,其多样化的临床表现以及远期并发症严重影响女性的身心健康和生活质量。为了深入认识PCOS,构建一个实用有效的动物模型十分必要。由于大鼠和小鼠体型小,繁殖周期短,具有较稳定的动情周期,对于实验研究既经济又方便,是最常用的实验动物之一。对国内外学者基于PCOS的发病特点、激素水平、内分泌及代谢指标建立的PCOS鼠类模型的研究进展予以综述,旨在评价各种模型的优缺点,为实验中模型的选择提供指导。

关键词: 多囊卵巢综合征, 疾病模型,动物, 鼠科, 基因编辑, 综述

Abstract:

Polycystic ovary syndrome（PCOS） is considered to be one of the most common female endocrine diseases with complex etiology and high incidence rate. Its diverse clinical manifestations and long-term complications seriously affect the physical and mental health and quality of life of patients. Therefore, the establishment of a practical and effective experimental model is very important to the research of PCOS. Rats and mice are one of the most commonly used experimental animals because of their small size, short reproductive cycle and stable estrous cycle. This paper reviews the research progress of mouse models of PCOS at home and abroad based on the clinical characteristics, hormone levels, endocrine and metabolic indexes of PCOS, aims to evaluate the advantages and disadvantages of various models and provide guidance for the selection of models in experiments.

Key words: Polycystic ovary syndrome, Disease models,animal, Muridae, Gene editing, Review

黄千千, 王娇剑, 宋殿荣. 多囊卵巢综合征鼠类动物模型的构建[J]. 国际妇产科学杂志, 2022, 49(3): 261-266.

HUANG Qian-qian, WANG Jiao-jian, SONG Dian-rong. Construction of Mouse Model of Polycystic Ovary Syndrome[J]. Journal of International Obstetrics and Gynecology, 2022, 49(3): 261-266.

图/表 1

参考文献 41

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造模方法	造模用药	鼠别	鼠龄	给药方式	剂量（时间）	造模周期（d）
雄激素造模法	DHEA	SD大鼠	23日龄	皮下注射	6 mg/100 g	20
	DHT	Wistar大鼠	5~9日龄	皮下注射	1.25 mg	35
	TP	SD大鼠	21日龄	皮下注射	2.5~10 mg	90
雌激素造模法	EV	SD大鼠	4~5周龄	皮下注射	2~4 mg	28
	EB	Wistar大鼠	2日龄	皮下注射	0.5 mg	90
芳香化酶抑制剂造模法	来曲唑	SD大鼠	6周龄	灌胃	1 mg/kg	21
孕激素造模法	孕激素+hCG	SD大鼠	23~24日龄	皮下埋植+皮下注射	左旋18-甲基炔诺酮硅胶棒3 mm/只+ hCG 1.5 IU/次,2次/d	12
胰岛素造模法	胰岛素+hCG	SD大鼠	6周龄	皮下注射	1.5~6.0 IU/d hCG+ 0.5~6 IU INS	22

造模方法	造模用药	鼠别	鼠龄	给药方式	剂量（时间）	造模周期（d）
雄激素造模法	DHEA	SD大鼠	23日龄	皮下注射	6 mg/100 g	20
	DHT	Wistar大鼠	5~9日龄	皮下注射	1.25 mg	35
	TP	SD大鼠	21日龄	皮下注射	2.5~10 mg	90
雌激素造模法	EV	SD大鼠	4~5周龄	皮下注射	2~4 mg	28
	EB	Wistar大鼠	2日龄	皮下注射	0.5 mg	90
芳香化酶抑制剂造模法	来曲唑	SD大鼠	6周龄	灌胃	1 mg/kg	21
孕激素造模法	孕激素+hCG	SD大鼠	23~24日龄	皮下埋植+皮下注射	左旋18-甲基炔诺酮硅胶棒3 mm/只+ hCG 1.5 IU/次,2次/d	12
胰岛素造模法	胰岛素+hCG	SD大鼠	6周龄	皮下注射	1.5~6.0 IU/d hCG+ 0.5~6 IU INS	22