Abstract: Objective： To detect specific serum biomarkers of adenomyosis by surface-enhanced laser ｄｅｓｏｒｐｔｉｏｎ／ｉｏｎｉｚａｔｉｏｎ ｔｉｍｅ－ｏｆ－ｆｌｉｇｈｔ ｍａｓｓ ｓｐｅｃｔｒｏｍｅｔｒｙ（ＳＥＬＤＩ－ＴＯＦ－ＭＳ） ｔｅｃｈｎｏｌｏｇｙ． Ｍｅｔｈｏｄｓ： Serum samples from women with adenomyosis were compared with normal control groups by SELDI-TOF-MS technology. After finding the biomarkers, the diagnostic models were evaluated and blinded test was performed to exam the sensitivity and specificity. Results：In this study, the difference between the adenomyosis and control groups was ｓｉｇｎｉｆｉｃａｎｔ ｆｏｒ １２ ｐｒｏｔｅｉｎ ｐｅａｋｓ（Ｐ＜０．０５）． Ａｍｏｎｇ ｔｈｅｍ ｔｗｏ ｐｒｏｔｅｉｎ ｐｅａｋｓ ｗｉｔｈ ｍ／ｚ ｏｆ ２．７４８ ｋu ａｎｄ ５．７５９ ｋu were protected be of high value in the diagnosis of adenomyosis. Then we set up the diagnostic model of ａｄｅｎｏｍｙｏｓｉｓ ｗｉｔｈ ｕｓａｇｅ ｏｆ ｔｈｅｓｅ ｔｗｏ ｐｒｏｔｅｉｎ ｐｅａｋｓ， ｔｈｅ ｓｅｎｓｉｔｉｖｉｔｙ ａｎｄ ｓｐｅｃｉｆｉｃｉｔｙ ｗｅｒｅ １００％ ａｎｄ ８０％． Ａｎａｌｙｓｉｓ of spectra from the completely blinded test set accurately classified with a sensitivity and specificity of 86% and 100%. Conclusions： SELDI-TOF-MS technology plays an important role on screening the diagnostic biomarker of adenomyosis. The pattern of combined markers provides a powerful and reliable diagnostic method for adenomyosis with high sensitivity and specificity.

Key words: Endometriosis, Proteomics, Diagnosis, Adenomyosis, Spectrometry, mass