Abstract: Objective：To analyze the serum biomarkers of the ovarian cancer patients, to establish serum diagnosis models and to find potential biomarkers with MALDI-TOF-MS combined with WCX magnetic bead. Methods：8 healthy controls, 22 benign ovarian tumor, 16 borderline tumor, and 16 ovarian cancer patients were enrolled to pre-extract the serum samples using the MB-WCX kit. Then, Biomarker Wizard software was used to detect protein peaks and potential difference between these groups. Last comparing with CA125 in sensitivity and specificity. Results：A diagnostic pattern for borderline tumor from healthy controls using m/z 4 508.71 was established with 100% sensitivity and 62.5% specificity, for borderline tumor from benign healthy controls was 83.3% sensitivity and 72.8% specificity, and m/z 2 665.32 for ovarian cancer from borderline tumor was established with 100% sensitivity and 50.0% specificity. Further compared to traditional CA125 (sensitivity 76%), the sensitivity of this methods is higher (compared with normal control group was 100%, compared with benign tumor group of 83.3%). Conclusions：MALDI-TOF-MS can be used not only to establish serum diagnosis models, but also to search for potential biomarkers in ovarian cancer patients′ serum samples.
【Keywords】 

Key words:  Ovarian neoplasms, Gas chromatography-mass spectrometry, Tumor markers, biological, Matrix-assisted laser desorption ionization time-of-flight mass spectrometry