Abstract: Objective：To investigate the feasibility of vaginoplasty with an acellular dermal matrix（ADM） patch in the treatment of Mayer-Rokitansky-Kuster-Hauser（MRKH） syndrome. Methods：The clinical data of 42 cases of congenital absence of vagina (MRKH syndrome) from January 2012 to April 2015 in our hospital were analyzed retrospectively，including 12 cases underwent ADM vaginoplasty（observer group） and 30 cases underwent laparoscopic peritoneal vaginoplasty（control group）. The perioperative, postoperative complications and postoperative follow-up data were compared between the two groups. Results：All cases were successful. No injury occurred arround the adjacent organs. The mean operation time，intraoperation blood loss，postoperative anal exhaust time and complications in observer group were less than that of control group（P＜0.05），while the hospitalization expenses in observer group was higher than that of control group（P＜0.05）. The follow-up showed that the width of artificial vagina of the two groups were 2 fingers in average and the length ＞8 cm with smooth and elastic mucosa. The vagina mucosa was formed 3 months in the observer group after vaginoplasty to start sexual life，while 6 months in the control group. The female sexual function index（FSFI） of the 8 cases in the observer group had no statistical difference with that of 16 cases in the control group（P＞0.05）. Conclusions：Both the ADM vaginoplasty and the laparoscopic peritoneal vaginoplasty are optimal procedures for vaginal reconstruction. However，the ADM vaginoplasty is simpler，less injury，fewer complications and earlier start sexual life.

Key words: Mayer-Rokitansky-Kuster-Hauser syndrome, Acellular dermal matrix patch, Vagina, Peritoneum