Abstract: Objective：To establish an animal model with ovarian carcinoma to simulate the clinical tumor development. Methods：Human ovarian carcinoma cell lines SKOV3 and multi-level A2780 in exponential phase of growth were inoculated subcutaneously in nude mice， A2780 cells were also inoculated intraperitoneally. The third generation of SKOV3 subcutaneous tumor was transplantated below the capsule of the right ovary. Results：Subcutaneous inoculations of human ovarian carcinoma SKOV3 cells and orthotopic transplantations of SKOV3 subcutaneous tumor both succeeded in all mice. Six to eight weeks after orthotopic transplantations， tumors invasion into surrounding tissues were observed， as well as dull red effusion with peritoneal metastasis and lymph node metastasis. The subcutaneous inoculation of multi-level cell lines A2780 all failed in tumor formation. Eight weeks after A2780 cells were injected in intraperitoneal cavity， 2-3 mL colorless ascites appeared， ovarian and spleen grew obviously， but tumor was not detected. Conclusions：The SKOV3 orthotopically transplantated animal model was successfully established. The inoculation of A2780 cell lines failed in tumor formation， possibly due to their sensitivities to non-specific immunity in the host.

Key words: Ovarian neoplasms, Carcinoma, Models, animal, Orthotopic transplantation, Lymphatic metastasis