Abstract: Objective：To investigate the gynecological clinic detection rate ，proportion and age distribution of vaginal microflora imbalance. Methods：Gram staining gynecological clinic patients with clinical symptoms of vaginal secretions， detection of vaginal pH. Results：Vaginal microbial disorder detection rate was 51.33%，vulvovaginal candidiasis accounted for 28.99%， bacterial vaginosis accounting for 8.57%，trichomoniasis vaginosis was 2.03%，mixed vaginitis was 0.48%，undefined vaginal microbial imbalance was 59.92%. Vaginal microbial disorders in patients composition of each age group：＜20 years old was 2.4%，21-30 age group was 32.7%，31-40 age group was 38.2%， 41-50 age group was 21.7%，＞50 years old group was 5.0%，all kinds of vaginal micro-ecological imbalance， the median age in addition to mixed vaginitis was in 21-30 years old， the other are in the 31-40 years of age. Conclusions：Vulvovaginal candidiasis were higher than bacterial vaginosis，trichomonas vaginitis and mixed vaginitis. Vulvovaginal candidiasis，bacterial vaginosis，trichomonas vaginitis and mixed vaginitis were predilection in reproductive age females. In addition to inflammation of the vagina， there are most of the vaginal microbial disorders need to define.

Key words: Candidiasis, vulvovaginal, Trichomonas vaginitis, Vaginosis, bacterial, Vaginitis