Abstract: Objective：Study Twist2 and E-cadherin expression in cervical intraepithelial neoplasia and cervical squamous cell carcinoma，and explore their roles and significance in the process of cervical cancer. Methods：Using western blot （Western blot） and immunohistochemistry method to detecte Twist2，E-cadherin expression in normal cervical tissue （control group），group CINⅠ，CINⅡ-Ⅲ group，cervical squamous cell carcinoma. Results：The differences of the positive expression rate of Twist2 and E-cadherin in four groups were statistically significant （χ2=48.163，P=0.000；χ2=43.169，P=0.000）. The positive expression rate of Twist2，E-cadherin in cervical squamous cell differentiation，clinical stage，lymph node metastasis between the groups，the difference was statistically significant （P＜0.05），no statistically significant difference between the different age groups（P＞0.05）. Twist2 and E-cadherin expression was negatively correlated （r=-0.401，P=0.003） in cervical squamous cell carcinoma. Control group，CIN group，cervical squamous cell carcinoma relative expression levels of Twist2，E-cadherin difference was statistically significant（F=652.225，P=0.000；F=299.112，P=0.000）. Conclusions：Twist2，E-cadherin may participate in the process of malignant transformation of cervical squamous cell carcinoma，joint detection Twist2，E-cadherin help to determine the degree of malignancy of cerical cancer and its prognosis.

Key words: Twist transcription factor, Cadherins, Uterine cervical neoplasms, Cervical intraepithelial neoplasia, Blotting, western