Abstract: Objective:This study investigated regional distribution in the contribution made by different human papilloma (HPV) types to invasive cervical cancer (ICC), and to analyze the relation of HPV and clinical biological behavior in cervical cancer in Xuzhou region. Methods: Using nucleic acid hybridization technique to estimate and statistic analysis the HPV prevalence through 142 women with ICC. Results: ①Among the 142 women with ICC, a total of 136 participants (95.78%) had HPV infection. 19 of the 136 women (13.97%) who developed ICC were multiple HPV types infection, and 117 of them (86.03%) demonstrated a single HPV infection(P＜0.01). ②The 13 most common HPV types were identified by this study. The 8 HPV types in single infection were, in order of decreasing prevalence, HPV16(66.18%), 18(6.62%), 31(5.88%), 58(2.21%), 45(2.21%), 33(1.47%), 6(0.74%) and CP8304(0.74%); other HPV types in mutiple infections were HPV 68(2.94%), 52(2.21%), 66(1.47%), 39(0.74%), 11(0.74%). HPV16 (77.94%) were found more commonly than other HPV types and these differences were statistically significant(P＜0.01). Total infection of HPV18 was 7.35%. These were no statistically significant between HPV18 and HPV31，58，33(P＞0.05). ③There was not considerable variation in the rate of HPV infection between different cervical cancer clinical stage and histopathological grading(P＞0.05). ④Multiple infections seemed to have no relation to risk of ICC, whereas, to be decreasing with increasing pathological grade. Conclusions: A majority of ICC was associated with single HPV infection and approximately HPV16 was the predominant type followed by HPV１８，31 and 58 in Xuzhou regions. Also there was not an increased risk of ICC associated with an abnormal cytology at baseline high-risk HPV types (HR-HPV) mutiple infections. HPV infection did not appear to be associated with progress of ICC and differentiation of the tumor cells.

Key words: Uterine cervical neoplasms, Carcinoma, Papillomaviridae, Genotype