Abstract: Objective： We tested the amplification of TERC gene and HPV infection at all grade cervical lesions combined with colposcopy，and discussed the clinical value. Methods：219 patients were preliminarily diagnosed as cervical lesions with colposcopy. hTERC gene amplification（n=219） was tested by Fluorescence in situ hybridization（FISH）， and HPV infection（n=132） by SPR assay. The results were statistically analyzed. Results：①59 cases were diagnosed CIN I， 76 cases CIN II， 56 cases CIN III and 28 cases cervical cancer. There had correlation between the colposcopic diagnosis and histological diagnosis（P<0.05）. ②The positive amplification rates of hTERC gene at all levels of cervical lesions were 7.9%， 10.9%， 44.6%， 63.2%， 82.6% respectively， the differences among the groups were statistically significant（P<0.000 1）. ③Among 132 patients， there were 54 cases of high-risk HPV infection， 29 cases of low-risk HPV infection， 49 case of HPV negative. There was a statistically difference between HPV Isoforms infection and cervical intraepithelial neoplasia（P<0.05）. ④At the same time， the hTERC gene and HPV were tested also. In 54 cases with infection of high risk HPV, 33 cases were with abnormal hTERC gene amplification. And in 27 cases for low-risk HPV there were 7 cases with abnormal hTERC gene amplification. There was statistically difference between groups（P<0.001）. Conclusions： Colposcopy， as one method for diagnosing early cervical lesions， maybe leads to missed diagnosis or over-diagnosis. High risk HPV subtype infection may be one of factors which lead to abnormal hTERC gene amplification. hTERC gene amplification and HPV infection is closely related to the progress of high-grade cervical lesions， which may be the direct cause of cervical cancer. Colposcopy combined with HPV and hTERC gene may be an effective means for diagnosing early cervical lesions.

Key words: Uterine cervical neoplasms, Cervical intraepithelial neoplasia, Colposcopy, Telomerase, Papillomaviridae