Abstract: Objective：To study the influence of different concentrations of cholesterol on cell apoptosis and producing uric acid of trophoblast cell. Methods：Primary culture trophoblasts cells （HPT-8） in the medium with different concentrations of cholesterol for 24 hours，then Annexin V/PI method were used to detect different cells′ apoptosis. Meanwhile，we used ELISA to detect uric acid concentration in the supernatant after 24 hours. Results：Through the analysis we found that the higher cholesterol concentrations （0，25，50，100 mg/L） in medium，the higher cell apoptosis rate. The level of uric acid in the cell culture supernatant is higher，and presents a dose dependent. Conclusions：When medium cholesterol concentration become high，it cause more serious apoptosis of cells，which leads to increased number of microparticles. At the same time the high cholesterol concentration can also cause increased production of uric acid，then induce system inflammatory response and endothelal dysfunction. The two factors play important roles in the pathogenesis of preeclampsia.

Key words: Pre-eclampsia, Cholesterol, Necrosis, Apoptosis, Uric acid