国际妇产科学杂志

国际妇产科学杂志 ›› 2013, Vol. 40 ›› Issue (5): 470-471.

• 论著 • 上一篇    下一篇

卵巢癌中微小RNA-210的表达及临床意义

鲍留平, 仲建新, 张 薇   

  1. 224600 江苏省响水县人民医院妇产科（鲍留平）；南通大学附属医院妇产科（仲建新）；解放军第一五七医院（张 薇）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-10-15 发布日期:2013-10-15

Expression and Clinical Significance of MicroRNA-210 in Ovarian Cancer

BAO Liu-ping，ZHONG Jian-xin，ZHANG Wei   

  1. Department of Obstetrics and Gynecology，Xiangshui Hospital of Jiangsu，Xiangshui County 224600，Jiangsu Province，China（BAO Liu-ping）；Department of Obstetrics and Gynecology，Affiliated Hospital of Nantong University，Nantong 226001，Jiangsu Province，China（ZHONG Jian-xin）；PLA 157 Hospital，Shenyang 110045，China（ZHANG Wei）
  • Received:1900-01-01 Revised:1900-01-01 Published:2013-10-15 Online:2013-10-15

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摘要： 目的：检测上皮性卵巢癌组织中微小RNA-210（miR-210）的表达水平，初步探讨miR-210在卵巢癌发病中的作用。方法：采用多聚腺苷酸加尾实时荧光定量反转录聚合酶链反应［ｐｏｌｙ（Ａ）－RT-qPCR]检测上皮性卵巢癌、良性上皮性卵巢肿瘤及正常卵巢组织标本中miR-210的表达。结果：miR-210在卵巢癌组中表达低于良性卵巢肿瘤组和正常卵巢组，3组间差异有统计学意义（F=893.213，P=0.000）；且随着卵巢癌组织临床分期及组织分化的进展，miR-210表达有下降的趋势。结论：miR-210在上皮性卵巢癌组织中低表达，在卵巢癌发生发展中发挥重要的抑癌基因作用，且与卵巢癌分期、组织分级及淋巴结转移密切相关。

关键词: 卵巢肿瘤, 微RNAs, 逆转录聚合酶链反应, 基因, 肿瘤抑制

Abstract: Objective：Detection the expression of miR-210 and investigation its roles in epithelial ovarian cancer（EOC）. Methods：Poly（Ａ）-RT-qPCR was used to detect the expression of miR-210 in epithelial ovarian cancers and benign epithelial ovarian cysts and normal ovarian tissues. Results：miR-210 in ovarian cancer group was lower than that of benign ovarian tumors and normal ovarian group，significant difference among the three groups（F=893.213，P=0.000）. With the progress of the clinical staging of ovarian cancer tissues and tissue differentiation，miR-210 expression there has a downward trend. Conclusions：Our data suggest that miR-210 expressed lowerly in EOC，it might play an important role as an anti-oncogene in the genesis and development of epithelial ovarian cancer. It is bound with lymphatic metastasis，stages of ovarian cancer and tissus grading.

Key words: Ovarian neoplasms, MicroRNAs, Reverse transcriptase polymerase chain reaction, Genes, tumor suppressor