关键词: 小鼠, SCID, 黄芪多糖, 子宫内膜肿瘤, 模型, 动物, 癌基因蛋白质类, 脂质运载蛋白

Abstract: Objective：To extract the active ingredients of Astragalus astragalus polysaccharide, observe the immune reconstruction load in endometrial carcinoma SCID mice serum neutrophil gelatinase associated lipocalin (neutrophil gelatinase-associated lipocalin, NGAL) and its receptor NGALR expression influence. Methods：36 female CB-17 SCID mice were divided into blank control group, tumor group and treatment group, tumor group and treatment group were intraperitoneal injection of 5×107/mL Lymphocytes Separation Medium (PBMC), lymphocyte suspension hind leg subcutaneously with 3×106/mL endometrial adenocarcinoma cell line (HEC-1B) establishment of human immune reconstitution in endometrial cancer bearing model. After treatment, the treatment group was injected with astragalus polysaccharide at 20 mg/kg dose for 10 days (d), and the normal control group and the tumor bearing group were injected with normal saline. Enzyme linked immunosorbent assay (ELISA) expression of serum NGAL and NGALR protein were determined by reverse transcriptase polymerase chain reaction (RT-PCR) expression of NGAL gene and NGALR gene detection of tumor tissue, measuring tumor group and treatment group tumor volume, tumor weight and tumor inhibition rate was calculated. Results：After treatment, the treatment group of NGAL and NGALR protein expression decreased, NGAL mRNA and NGALR mRNA were low expression, tumor volume and tumor weight were significantly decreased, the inhibition rate increased, compared with the tumor bearing group and the same group before treatment, the differences were statistically significant (P＜0.05). Conclusions：Astragalus polysaccharides may exert antitumor effects by inhibiting the expression of NGAL and NGALR proteins and their genes.

Key words: Mice, SCID, Astragalan, Endometrial neoplasms, Models, animal, Oncogene proteins, Neutrophil gelatinase associated carrier protein