3D生物打印的子宫内膜再生细胞生物支架用于宫腔粘连的探讨

doi:10.12280/gjfckx.20210456

摘要/Abstract

摘要：

现有的治疗宫腔粘连的方法存在局限性,复发率高,妊娠率低。对子宫内膜再生和功能的恢复几乎无帮助,目前还没有针对该问题最佳的治疗策略。近年来,干细胞成为治疗宫腔粘连的研究热点,而如何维持细胞在体内的生物学活性成为关键问题。3D生物打印技术能够在移植体内模拟细胞体外生存微环境,为移植细胞发挥治疗效应提供了有效的保障。目前已从女性月经血中获得了具有干细胞特征的子宫内膜再生细胞（endometrial regenerative cells,ERC）,发现其增殖能力极强、具有强大的多向分化潜能。但是否可以通过移植应用3D生物打印技术构建的ERC生物支架治疗宫腔粘连至今尚无报道。综述3D生物打印的关键技术、细胞和生物墨水,以及3D生物打印的子宫内膜再生细胞生物支架在治疗宫腔粘连中应用的可行性和局限性。

关键词: 生物打印, 打印,三维, 子宫疾病, 组织黏连, 干细胞, 3D生物打印技术, 子宫内膜再生细胞, 宫腔粘连, 生物墨水

Abstract:

The existing treatment of intrauterine adhesions has limitations, such as high recurrence rate and low pregnancy rate. There is little help for endometrial regeneration and functional recovery, and there is no optimal treatment strategy for this problem. In recent years, stem cells have become a research focuses in the treatment of intrauterine adhesions, and how to maintain the biological activity of cells in vivo has become a key issue. 3D bio-printing technology can simulate the microenvironment of the transplanted cells in vitro, providing an effective guarantee for the therapeutic effect. At present, endometrial regenerative cells (ERC) with the characteristics of stem cells have been obtained from female menstrual blood, and found to have strong proliferative ability and multidirectional differentiation potential. However, it has not been reported whether ERC bio-scaffolds constructed by 3D bioprinting technology can be transplanted to treat intrauterine adhesions. This review briefly introduced the key technologies, cells and bio-inks of 3D bioprinting, and prospected the feasibility and limitations of the application of 3D bioprinted endometrial regenerative cell bioscaffolds for the treatment of intrauterine adhesions.

Key words: Bioprinting, Printing,three-dimensional, Uterine diseases, Tissue adhesions, Stem cells, 3D bioprinting technology, Endometrial regenerative cells, Intrauterine adhesions, Bio-ink

夏瑞琦, 孔宪超. 3D生物打印的子宫内膜再生细胞生物支架用于宫腔粘连的探讨[J]. 国际妇产科学杂志, 2022, 49(1): 111-115.

XIA Rui-qi, KONG Xian-chao. Application of 3D Bioprinted Endometrial Regenerative Cell Biological Scaffold in Intrauterine Adhesions[J]. Journal of International Obstetrics and Gynecology, 2022, 49(1): 111-115.

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打印技术	材料黏度（mPa/s）	打印速度	细胞活力	费用	细胞密度
喷墨式生物打印^[9]	3.5~12	快	>85%	低	低,<10⁶个细胞/mL
挤出式生物打印^[8]	30~>6×10⁷	中等	40%~80%	中等	高,细胞球状体
激光辅助生物打印^[10]	1~300	慢	>95%	高	中等,10⁸个细胞/mL

打印技术	材料黏度（mPa/s）	打印速度	细胞活力	费用	细胞密度
喷墨式生物打印^[9]	3.5~12	快	>85%	低	低,<10⁶个细胞/mL
挤出式生物打印^[8]	30~>6×10⁷	中等	40%~80%	中等	高,细胞球状体
激光辅助生物打印^[10]	1~300	慢	>95%	高	中等,10⁸个细胞/mL