Abstract:

Objective: To investigate the effect of uterine microenvironment on early pregnancy in patients with recurrent spontaneous abortion (RSA). Methods: From October 2021 to January 2022, intrauterine lavage was collected from RSA patients who were admitted to our hospital for uterine clear operation and normal early pregnant women who were admitted for induced abortion during the same period. Concentrations of lipopolysaccharide (LPS), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2) and interferon-γ (IFN-γ) were detected. Endometrial cells and extravillous trophoblast cells were respectively interfered with intrauterine lavage to detect mRNA expression of TNF-α, IL-2, IFN-γ in endometrial cells and invasion and migration ability of extravillous trophoblast cells. Results: The concentrations of LPS, TNF-α, IL-2 and IFN-γ in intrauterine lavage of RSA patients were significantly higher than those of normal women in early pregnancy (P<0.05). The mRNA expressions of pro-inflammatory factors TNF-α, IL-2 and IFN-γ in endometrial cells of RSA patients were significantly increased after intrauterine lavage intervention compared with those of normal women in early pregnancy (P<0.05). After the intervention of intrauterine lavage with extravillous trophoblast cells in RSA patients, the number of invasive cells and migration area of cells were significantly decreased compared with that of normal women in early pregnancy (P<0.05). Conclusions: The uterine cavity of patients with RSA presents a microinflammatory state, which affects the invasion and migration ability of extravillous trophoblast cells and is not conducive to the maintenance of pregnancy.

Key words: Abortion, habitual, Pregnancy, Cellular microenvironment, Cytokines, Intrauterine lavage