Abstract: Objective： To explore the effects of eukaryotic vector expressing short hairpin RNA （shRNA） targeting signal transducers and activators of transcription 3（STAT3） on the location and metastasis of human cervical carcinoma SiHa cells. Methods： The shRNA templates was designed based on STAT 3 gene sequence and was cloned into pSilencer2.1-U6-neo vector. The resultant plasmid was transfected into SiHa cells with Lipofectamine 2000. The Western blot and RT-PCR were used to detect STAT3 protein and mRNA respectively. The colony formation assay and Transwell cabin assay were performed to measure the location and metastasis of SiHa cells. Results： The plasmid pSTAT3-siRNA was successfully constructed and transfected into SiHa cells. The expressions of STAT3 in SiHa cells decreased. At the same time， the number of colony formation in soft agar and cells penetrating matrigel also decreased. Conclusions： The STAT 3 specific shRNA expression vector could inhibit the cellular location and metastasis of cervical cancer cells through suppressing the STAT 3 expression.

Key words: Uterine cervical neoplasms, RNA interference, Neoplasm invasiveness, Reverse transcriptase polymerase chain reaction, Signal transducers and activators of transcription 3