Abstract: Objective：To explore risk assessment of cervical lesion and guidance of the best clinical triage management and treatment by combined detection of human papillomavirus L1 capsid protein and human papillomavirus（HPV） genotyping，thinprep cytology test（TCT）. Methods：Retrospective analysis of 1 593 women of cervical cancer screening in the gynecological
clinic of the Second Affiliated Hospital of Zhengzhou University from September 2010 to December 2011，and TCT and genotyping of HPV-DNA testing at the same time，in which 592 patients who was HPV-positive or TCT positive or both abnormal were sent to colposcopic biopsy for pathological examination and who was HPV-DNA typing-positive were detection of HPV L1 capsid protein expression. Results：TCT combined with HPV-DNA detection in cervical intraepithelial neoplasia Ⅲ（CIN Ⅲ） and the above cases had the highest rate and reached 100% positivity in squamous cell carcinoma（SCC）；the positive rate of HPV L1 capsid protein expression show a decreased trend with the increasing level of cervical lesions，the expression of HPV L1 capsid protein and SCC was 0. The positive expression rate of HPVL1 capsid protein in each group was significantly different （P＜0.05）. Conclusions：It is an essential indicator that TCT combined HPV genotyping in cervical lesions screening，and HPVL1 capsid protein detection had important guiding value on risk assessment of cervical lesions. Three factors effective combination can be timely and accurate diversion and treatment of patients with cervical lesions.

Key words: Papillomaviridae, Uterine cervical neoplasms, Cervical intraepithelial neoplasia, Biopsy, Cervix uteri