Abstract: Objective： To explore serum human epididymis protein 4 （HE4），cancer antigen 125 （CA125） for the dualistic model about epithelial ovarian cancer （EOC） application value. Methods：The electrochemical luminescence method to detect serum HE4 and CA125 levels of patients in hospital to be intend to do surgery because of pelvic masses or ovarian cyst from February 2012 to April 2013，including gynecological inflammation（n=65），endometriosis （n=509），benign ovarian tumors（n=887），borderline ovarian tumors（n=73），epithelial ovarian cancer（n=132，78 cases of Ⅰtype，54 cases of Ⅱ type） and 71 cases of healthy women，to evaluate in application value of the model. Results：Epithelial ovarian cancer patients serum HE4 and CA125 expression level and FIGO stage were a significant positive correlation（rs=0.534，0.541，P=0.000）. The serum level of HE4 and CA125 expression in the epithelial ovarian cancer of Ⅱtype were higher than that of the Ⅰ type in all stage，the difference was statistically significant. The serum level of HE4 expression in the high-grade serous carcinoma were higher than the low-grade serous carcinoma，the difference was statistically significant. The serum level of HE4 expression in epithelial ovarian cancer were higher than that of the other groups，the difference was statistically significant（P＜0.05）. The serum level of CA125 expression in Ⅰstage EOC and gynecological inflammation，endometriosis were no significant difference（P＞0.05）. The serum level of CA125 expression in Ⅱ-Ⅲstage EOC of Ⅰ type and endometriosis were no significant difference （P＞0.05）. The area under the receiver operating curve （ＲＯＣ－ＡＵＣ） of HE4 and ROC were 0.877，0.786，accuracy（90.0%，70.5%），sensitivity（67.4%，69.7%），specificity（92.1%，72.6%），Youden index（0.595，0.403）. Conclusions：The specificity of HE4 assay is higher than CAl25 assay in the diagnosis of ovarian carcinoma. Single serum HE4 and CA125 detection has a certain significance to the early diagnosis staging，pathology typing and prognosis of epithelial ovarian cancer，which can be used as an important index for the diagnosis of epithelial ovarian cancer.

Key words: Tumor markers, biological, CA-125 antigen, Diagnosis, Human epididymis protein 4, Dualistic model