Abstract: Objective： To investigate the effect of the miR-200c over-expression on the ability of proliferation, migration and invasion in OVCAR-3 ovarian cancer cells. Methods：Constructed retrovirus expression vector pMSCVpuro-miR-200c and transfected OVCAR-3 cells to overexpress miR-200c. By using CCK8 and Transwell methods to detect the ability of OVCAR-3 cells in proliferation and metastasis. Different expression genes involved in OVCAR-3 cells were detected by using gene chip before and after transfected with pMSCVpuro-miR-200c. Results：Compared with untransfected cells, the ability of proliferation, migration and invasion in pMSCVpuro-miR-200c transfected OVCAR-3 cell are significantly decreased（P＜0.01）. 190 up-regulated genes and 166 down-regulated genes were detected in OVCAR-3 cells after over-expressed miR-200c. Conclusions：As a tumor suppression gene，overexpression of miR-200c could inhibit the proliferation，migration and invasion of ovarian cancer cells.

Key words: Ovarian neoplasms, Cell proliferation, Cell movement, Neoplasm invasiveness, miR-200c