Abstract: Objective：To detect treatment effect on cervical cancer side population cells by nano-molecular polyamide-amine （PAMAM）-mediated drug resistance gene ATP-binding cassette superfamily G member 2（ABCG2） silencing. Methods：The cervical cancer side population cells were isolated by flow cytometry assay from cervical cancer cells. The protein level of ABCG2 was detected by immunofluorescence method. MTT assay was used to detect the inhibition rate of tumor cells induced by doxorubicin. According to the experimental requirements, the three groups, siRNA group, antisense group and control group are set up. After the design and synthesis of siRNA targeting ABCG2 gene，it was transfected into tumor cells by PAMAM，then the ABCG2 gene expression was detected by western blot，and the restore of tumor cells to doxorubicin sensitivity by detected by MTT assay. Finally，these cells were transplated into the subcutaneous of de-thymus mice and the tumor volume was detected. Results：The protein level of ABCG2 in cervical cancer side population cells was higher than that of nonside population cells（P＜0.05）；The inhibition rate of doxorubicin to non side population cell was significantly higher than that of side population cells （P＜0.01） by MTT，after ABCG2 gene silence，the inhibition rate in siRNA group recoverd to（69.4±11.3）%, showing significant differences with the control group and the non sense group（P＜0.01）. The animal experimental results show that the tumor volume in siRNA treatment group was significantly lower than the control group and antisense sequence group（P＜<0.05）. Conclusions：PAMAM can induce ABCG2 gene silencing in cervical cancer side population cell mass，to restore its treatment sensitivity to chemotherapeutic agents.

Key words: Uterine cervical neoplasms, Neoplastic stem cells, Drug resistance, neoplasmRNA, small interfering