Abstract: 【Abstract】  Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a kind of mass spectrometry technology, and it has successfully detected many new biomarkers, which is related to diseases, especially in the aspects of diagnosis and treatment of tumor，moreover it can quickly detect specific proteins expressed in cancer patients, aid to set specific treatments, and predict the outcome of treatment and the possibility of recurrence. In recent years, domestic and foreign scholars have carried out a number of MALDI-TOF-MS study ，which is about the analysis of differential promoetic and the detection of molecular protein related to drug resistance in ovarian cancer. The origin and present situation of MALDI-TOF-MS technology，the research progress in the diagnosis and treatment of ovarian cancer, the challenges and application prospect, will be discussed in this paper. These will provid new ideas and methods for ovarian cancer prevention, diagnosis and treatment.

Key words: Matrix assisted laser dissociation ionization time of flight mass spectroｍｅｔｒｙ, Ｓｐｅｃｔｒｏｍｅｔｒｙ, ｍａｓｓ, ｍａｔｒｉｘ－assisted laser desorption-ionization, Mass spectrometry, Ovarian neoplasms, Biological markers