Abstract: Objective：ABC294640 has shown its unique efficacy in the treatment of cancer. The purpose of this study was to investigate the inhibitory effect of ABC294640 on invasive and metastatic abilities of ovarian cancer cells and to explore its mechanism. Methods：Transwell experiments were preformed to detect the effect of ABC294640 on invasion and migration of ovarian cancer cells, and the regulatory effect of ABC294640 on the expression of c-Myc protein in ovarian cancer cells was analyzed by Western blotting. An intra-abdominal xenograft tumor model of ovarian cancer was set up to observe the effect of ABC294640 on the metastasis in vivo. Results：The ability of invasion and metastasis of ovarian cancer cells were significantly inhibited after the treatment with 50 μmol ABC294640 and the expression of c-Myc protein in ovarian cancer cells significantly decreased (P＜0.01). In the intra-abdominal xenograft tumor model of ovarian cancer in nude mice, the tumor number of abdominal metastases in ABC294640 group was significantly reduced compared with the control group. The expression level of c-Myc in tumor tissue was significantly lower than that in control group (P＜0.01). Conclusions：ABC294640 inhibited the invasion and migration of ovarian cancer cells, and its anti-invasion and metastasis mechanism might be related to the down-regulation of c-Myc protein expression.

Key words: Ovarian neoplasms, Neoplasm invasiveness, Neoplasm metastasis, Proto-oncogene proteins c-myc