Application of 3D Printed Hydrogel Stents in Prevention and Treatment of Intrauterine Adhesion

doi:10.12280/gjfckx.20231104

Abstract

Abstract:

Intrauterine adhesion (IUA) often leads to amenorrhea, infertility, and recurrent miscarriage, seriously affecting the physical and mental health of women. Clinically, surgical separation is the main treatment for IUA, but this approach frequently results in postoperative recurrence and has poor treatment efficacy. 3D bioprinted scaffolds have emerged as a cutting-edge approach in the treatment of IUA, capable of separating damaged areas to prevent adhesion formation and undergoing controllable in vivo degradation without the need for surgical removal. These scaffolds can be used for drug loading, stem cell transplantation, and exosome delivery, enabling the release of drugs or growth factors to the injured area to promote tissue repair and regeneration. Transplanting 3D bioprinted hydrogels loaded with stem cells or stem cell-derived exosomes to the damaged area can effectively promote the repair of IUA and improve pregnancy rates. 3D bioprinting can provide personalized treatment plans for patients, and the combined treatment strategy with stem cells or stem cell-derived exosomes holds promise as a powerful tool for treating IUA.

Key words: Intrauterine adhesion, Printing, three-dimensional, Stem cells, Therapy, Endometrial repair

SHI Ming, SUN Ming-hui, YANG Wei-wei, HUANG Hui-hua, ZHANG Chang-lin, David YB Deng. Application of 3D Printed Hydrogel Stents in Prevention and Treatment of Intrauterine Adhesion[J]. Journal of International Obstetrics and Gynecology, 2024, 51(3): 290-296.

Figures/Tables 3

References 34

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序号	水凝胶材料	参数	优点	缺点
1	GelMA、SA^[9]	10%GelMA，2%SA，1%光引发剂	多孔性、可压缩性高、生物相容性高	GelMA体内降解较慢、单独支架促修复效果有限
2	Gel、SA^[10]	20%Gel和4%SA，2∶1体积比，4度打印，10%氯化钙交联	可在体外体内长期支持负载细胞的活性，体内可自然降解，生物相容性高	人的诱导性多能干细胞移植到大鼠存在免疫反应，子宫内膜修复疗效有限
3	自交联HA^[11]	5 mg/mL	为UC-MSCs移植提供载体，防止细胞流出，提供物理支持	单独支架移植未改善恒河猴的宫腔形态及月经周期
5	Ⅰ型胶原蛋白^[12]	0.5%胶原冻干，105 ℃热脱水15 h	具有细胞外基质同样的纤维结构、具有多孔结构，适合MSCs的负载黏附和营养支持	需要和干细胞联合使用才能达到促修复和改善妊娠效果
6	壳聚糖^[13]	2%壳聚糖/乙酸溶液3 mL加入1.5 mL交联液，搅拌15 min，37 ℃交联形成水凝胶	生物相容性高，具备抗菌性能，稳定性高	制备过程较为复杂，氨基成分易被化学修饰，机械强度弱

序号	水凝胶材料	参数	优点	缺点
1	GelMA、SA^[9]	10%GelMA，2%SA，1%光引发剂	多孔性、可压缩性高、生物相容性高	GelMA体内降解较慢、单独支架促修复效果有限
2	Gel、SA^[10]	20%Gel和4%SA，2∶1体积比，4度打印，10%氯化钙交联	可在体外体内长期支持负载细胞的活性，体内可自然降解，生物相容性高	人的诱导性多能干细胞移植到大鼠存在免疫反应，子宫内膜修复疗效有限
3	自交联HA^[11]	5 mg/mL	为UC-MSCs移植提供载体，防止细胞流出，提供物理支持	单独支架移植未改善恒河猴的宫腔形态及月经周期
5	Ⅰ型胶原蛋白^[12]	0.5%胶原冻干，105 ℃热脱水15 h	具有细胞外基质同样的纤维结构、具有多孔结构，适合MSCs的负载黏附和营养支持	需要和干细胞联合使用才能达到促修复和改善妊娠效果
6	壳聚糖^[13]	2%壳聚糖/乙酸溶液3 mL加入1.5 mL交联液，搅拌15 min，37 ℃交联形成水凝胶	生物相容性高，具备抗菌性能，稳定性高	制备过程较为复杂，氨基成分易被化学修饰，机械强度弱