Journal of International Obstetrics and Gynecology

Journal of International Obstetrics and Gynecology

   

Expressions of iNOS and Caspase 3 in Chorionic Tissues and Decidual Tissues of Patients with Embryonic Diapauses

ZHENG Jin-yan, LIAN Li-fen, HOU Hai-yan, LIANG Jing, CHEN Jun, CHEN Ya-qiong, DONG Qu-long   

  1. Tianjin Beichen Hospital, Tianjin 300400, China（ZHENG Jin-yan）；Department of Obstetrics and Gynecology, Affiliated Hospital of Logistics University of the Chinese People′s Armed Forces, Tianjin 300162, Chinａ（ZHENG Jin-yan, HOU Hai-yan, CHEN Jun, CHEN Ya-qiong, DONG Qu-long）；Maternity and Child Care Hospital of Qinhuangdao, Qinhuangdao 066000, Chinａ（LIAN Li-feｎ）；South of Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 102618, Chinａ（ＬＩＡNG Jinｇ）
  • Published:2017-06-15 Online:2017-06-15
  • Contact: CHEN Ya-qiong, E-mail：chenyq82@hotmail.com

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Abstract: Objective：To study the expression and the clinical importance of the iNOS and caspase 3 in the chorionic and decidual tissues of the patients whose embryos stop developing. Methods：30 cases of early unexplained embryonic diapauses patients (case group) and 30 normal early pregnant women (control group) for the study, chorionic and deciduas tissues caspase 3 and iNOS levels were tested by RT-PCR method, chorionic and decidual caspase 3 protein expression levels were tested by Western blot. Results：①In the chorionic and decidual tissues, caspase 3 mRNA, the total caspase 3 protein and caspase 3 fragment protein expression levels in the case group was significantly higher than the control group（P＜0.01）. ② In the chorionic and decidual tissues, iNOS mRNA expression level in the case group was significantly lower than the control group（P＜0.01）. Conclusions：In the chorionic and decidual tissues，iNOS and caspase 3 positive expression in the normal pregnancy and in the patients whose embryos stop developing. The iNOS and caspase 3 may involve in apoptosis, and then cause embryo stopping development.

Key words:  Embryonic diapauses, Nitric oxide synthase, Caspase 3, Apoptosis