Abstract: Objective：To investigate the effect of cinobufagin on the invasive growth of the endometrial carcinoma HHUA cells cultured in vitro. Methods：Endometrial carcinoma HHUA cells were cultured in vitro；after the action of cinobufagin with different concentration，MTT method was used to detect the proliferation of HHUA cells，the cellular apoptosis was tested by flow cytometry，the expression of CD44s was measured by RT-PCR，the ability of invasion was compared with transwell chamber model. Results：Endometrial carcinoma HHUA cells expressed CD44s mRNA of （1.31±0.25），number of invasive cell was 60.50±2.52；0.25 g/L cinobufagin didn't affect CD44s and invasive cell（P＞0.05）； after the action of 2.5 g/L cinobufagin，the expression of CD44s was 1.02±0.23， number of invasive cell was（30.50±3.95），these deta were different from the control group of endometrial carcinoma HHUA cells significantly（P＜0.05）；after the action of 25 g/L cinobufagin，the expression of CD44s was 0.97±0.39，number of invasive cell was 22.50±4.22，these data were different from the control group of endometrial carcinoma HHUA cells significantly（P＜0.05）；the action of 25 g/L cinobufagin was similar to that of 0.25 g/L cinobufagin（P＞0.05）. Conclusions：2.5 g/L cinobufagin inhibit the expression of CD44s mRNA and inhibit the invasive growth of the endometrial carcinoma HHUA cells.

Key words: Cinobufagin, Endometrial neoplasms, Genes, Apoptosis, Therapy