国际妇产科学杂志

国际妇产科学杂志 ›› 2012, Vol. 39 ›› Issue (3): 281-283.

• 论著 • 上一篇    下一篇

丝蛋白涂层轻质聚丙烯网片对兔腹壁疝模型组织学反应的影响

张 荣 , 吴氢凯, 罗来敏   

  1. 200233 上海交通大学附属第六人民医院
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-06-15 发布日期:2012-06-15
  • 通讯作者: 吴氢凯

Histologic Response of Silk Protein-coated and Noncoated Low-weight Polypropylene in an Abdominal Hernia Rabbit Model

ZHANG Rong，WU Qing-kai，LUO Lai-min   

  1. Shanghai Jiaotong University Affiliated Sixth People's Hospital，Shanghai 200233，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-06-15 Online:2012-06-15
  • Contact: WU Qing-kai

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被引次数

3

摘要： 目的：探讨宿主对有丝蛋白涂层和无丝蛋白涂层的2种不同轻质聚丙烯网片组织学反应的差别。方法：2种聚丙烯网片（Gynemesh网片和法国G819P网片）的有丝蛋白涂层和无丝蛋白涂层网片各6片，共计24片，裁剪为30 mm×30 mm大小。6只新西兰大白兔，每只均制作6个腹壁缺损，并分别植入有丝蛋白和无丝蛋白涂层Gynemesh?誖网片和有丝蛋白和无丝蛋白涂层G819P网片各1片，另2个腹壁缺损作为空白对照。植入后30，60和90 d时各处死2只动物，取出包括网片在内的腹壁全层，做大体观察、组织学观察以及免疫组化研究，包括炎症、纤维化反应、网片周围血管化反应以及胶原形成等。结果：所有网片均未发生侵蚀。有丝蛋白涂层网片组炎症反应和纤维化反应较轻，网片与组织融合良好。无涂层组可见少量的凋亡和坏死。结论：有丝蛋白涂层聚丙烯网片引起实验动物的组织学反应较轻，可以降低早期炎症反应和纤维化程度，减少组织粘连，降低网片相关并发症的产生。

关键词: 骨盆底, 脱垂, 腹壁, 丝素蛋白质类, 聚丙烯, 材料试验, 生物相容性材料, 兔

Abstract: Objective：The purpose of this study was to determine whether the host tissue response in the abdominal hernia rabbit model differs in 2 polypropylene meshes（coated and noncoated）. Methods：24 piece of two different kind polypropylene meshes Gynemesh?誖 and Aspide?誖G819P mesh （coated and noncoated，6 for each Group） 30 mm×30 mm size for each were surgically implanted in six abdominal hernia rabbit model which had six abdominal wall defects for each. Four abdominal wall defects for Coated/noncoated Gynemesh?誖 and G819P mesh，two left as the control. Animas were killed 30（n=2），60（n=2）and 90（n=2） days after surgery. Full-thickness sections of abdominal wall were evaluated included macroscopical examination，histological and immunohistochemical analysis，for example，inflammation，neovascularization，fibroblastic proliferation and collagen formation. Results：Erosion of meshes did not occur in all animals. silk protein-coated low-weight polypropylene meshes induced a mild inflammatory response with minimal fibrosis and good host tissue incorporation within the grafts. Few apoptotic and necrotizing cell were found in noncoated meshes. Conclusions：Coated meshes elicit a mild foreign body reaction，minimal fibrotic response and good host tissue incorporation within the grafts，reducing the inflammatory reaction，fibrosis，tissue adhesion and the generation of mesh-related complication.

Key words: Pelvic floor, Prolapse, Abdominal wall, Fibroins, Polypropylenes, Materials testing, Rabbits