关键词: 卵巢癌, 蛋白酶体抑制剂, MG132, 凋亡, 自噬

Abstract: Aim: To observe the growth of ovarian cancer SKOV3 cells after treated with proteasome inhibitor MG132, and the of autophagic and apoptotic factors, to investigate the inhibited mechanism of MG132?affected on ovarian?cancer cells. Method:Observation of SKOV3 cells after treated with MG132 at the concentrations of 0.5μg/ml, 1.5μg/ml, 2.5μg/ml, 3.5μg/ml. The growth of?cells were detected by MTT assay after treated with MG132 ; Apoptotic rates of cells were detected by flow cytometry(FCM) after treated with MG132; The of autophagic and apoptotic factors in cells were detected by IHC, western blot and RT-PCR. Results: MTT assay demonstrated the growth of SKOV3 cells were inhibited after treated with MG132 with concentration and time dependent.;FCM demonstrated the apoptotic rates?were gradually increased with the??increased MG132 concentrations and time.IHC?detected Beclin1 and Caspase3 were positive; Western blot detected Caspase3, Bim, Bax, Beclin1 were high expressed and Bcl-2 was low expressed in SKOV3 cells after treated with MG132, the protein of Beclin1, Caspase3, Bim, Bax were increased with the?increased MG132 concentrations and positive correlation, the protein of Bcl-2 was reduced and negative correlation with concentrations dependent; RT-PCR detected the mRNA relative quantity of Beclin1 and Caspase3 in MG132 groups were all higher than the control group ( P＜0.05 ) .Conclusion: The growth of ovarian carcinoma SKOV3 cells can be inhibited by proteasome inhibitor MG132 with concentration and time dependent. The inhibition effect is not only related with apoptosis, but also with autophagy.

Key words: ovarian cancer, proteasome inhibitor, MG132, apoptosis, autophagy