萝卜硫素的生物学效应及其在女性生殖系统中的研究进展

国际妇产科学杂志 ›› 2019, Vol. 46 ›› Issue (2): 216-219.

萝卜硫素的生物学效应及其在女性生殖系统中的研究进展

吴昊，卢晓声，吕杰强

325000 温州医科大学（吴昊）；温州医科大学附属第二医院育英儿童医院生殖中心（卢晓声，吕杰强）

收稿日期:2018-08-31 修回日期:2018-12-24 出版日期:2019-04-15 发布日期:2019-04-15
通讯作者: 吕杰强，E-mail：jieqianglu@126.com E-mail:jieqianglu@126.com
基金资助:
浙江省自然科学基金（LQ17H040003）

Biological Effects of Sulforaphane and It′s Role in Female Reproductive System

WU Hao, LU Xiao-sheng, LYU Jie-qiang

Wenzhou Medical University，Wenzhou 325000, Zhejiang Province, China （WU Hao）; Reproductive Center, The Second Affiliated Hospital & Yuying Children′s Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China（LU Xiao-sheng, LYU Jie-qiang）

Received:2018-08-31 Revised:2018-12-24 Published:2019-04-15 Online:2019-04-15
Contact: LYU Jie-qiang, E-mail: jieqianglu@126.com E-mail:jieqianglu@126.com

摘要/Abstract

摘要： 萝卜硫素（1-异硫氰酸-4-甲磺酰基丁烷，sulforaphane，SFN），又名莱菔子素，是一种提取自西兰花属植物的生物活性物质，是迄今发现的抗氧化能力较强的物质之一。当机体发生氧化应激刺激时，萝卜硫素可以通过活化核因子E2相关因子2（Nrf2）/抗氧化反应元件（ARE）通路，诱导多种抗氧化蛋白和解毒酶的表达，从而起到抗氧化效应。萝卜硫素还可与其他细胞靶点直接反应，从而发挥相应生物学效应，如诱导细胞凋亡、调节炎性介质等。因此，萝卜硫素已在多种疾病中被研究及使用，包括消化系统、呼吸系统、心血管系统和肿瘤等。女性生殖系统的抗氧化研究是目前的热点之一，而萝卜硫素在该系统的研究较少。现就萝卜硫素的基本结构、生物学效应以及其在女性生殖系统中的研究进展进行综述。

关键词: 萝卜硫素, 丁烷类, 泌尿生殖系统, 氧化性应激, Nrf2/ARE信号通路

Abstract: Sulforaphane (1-isothiocyanate-4-methanesulfonylbutane, SFN), also known as raphanin, is a bioactive substance extracted from broccoli plants, and is one of the strong antioxidant found so far. Once oxidative stress stimulus occurs, sulforaphane can induce the expression of various antioxidants and detoxifying enzymes by activating the Nrf2/ARE pathway to exert an antioxidant effect. The sulforaphane can also directly react with other cellular targets to exert biological effects such as inducing apoptosis and regulating inflammatory mediators. Sulforaphane has been used as an antioxidant in various disease research, such as digestive disease, respiratory diseases, cardiovascular diseases and tumors. However, there are few studies on its function in the female reproductive system. This article reviews the basic structure, biological effects and research progress of sulforaphane in female reproductive system.

Key words: Sulforafan, Butanes, Urogenital system, Oxidative stress, Nrf2/ARE pathway

吴昊，卢晓声，吕杰强. 萝卜硫素的生物学效应及其在女性生殖系统中的研究进展[J]. 国际妇产科学杂志, 2019, 46(2): 216-219.

WU Hao, LU Xiao-sheng, LYU Jie-qiang. Biological Effects of Sulforaphane and It′s Role in Female Reproductive System[J]. Journal of International Obstetrics and Gynecology, 2019, 46(2): 216-219.

参考文献

[1]Liu H, Talalay P.Relevance of anti-inflammatory and antioxidant activities of exemestane and synergism with sulforaphane for disease prevention[J].Proc Natl Acad Sci U S A, 2013, 110(47):19065-19070 [2]Tortorella S M, Royce S G, Licciardi P V, et al.Dietary Sulforaphane in Cancer Chemoprevention: The Role of Epigenetic Regulation and HDAC Inhibition[J].Antioxidants & Redox Signaling, 2015, 22(16):1382- [3]Ahn Y H, Hwang Y, Liu H, et al.Electrophilic tuning of the chemoprotective natural product sulforaphane[J].Proc Natl Acad Sci U S A, 2010, 107(21):9590-9595 [4]Bai Y, Chen Q, Sun Y P, et al.Sulforaphane Protection against the Development of Doxorubicin-Induced Chronic Heart Failure is associated with Nrf2 Upregulation[J]. [J].Cardiovascular Therapeutics, , 2017, 35:- [5]Jiang T, Tian F, Zheng H, et al.Nrf2 suppresses lupus nephritis through inhibition of oxidative injury and the NF-κB-mediated inflammatory response[J].Kidney International, 2014, 85(2):333-343 [6]Wang A S, Xu Y, Zhang Z W, et al.Sulforaphane protects MLE-12 lung epithelial cells against oxidative damage caused by ambient air particulate matter[J].Food & Function, 2017, 8(12):4555- [7]Myzak M C, Dashwood R H.Chemoprotection by sulforaphane: keep one eye beyond Keap1[J].Cancer Letters, 2006, 233(2):208-218 [8]姚丹燕, 吴秋云, 李倩.萝卜硫素调控机制的研究进展[J].园艺学报, 2014, 41(5):1020-1026 [9]Talalay P, Dinkova-Kostova A T, Holtzclaw W D.Importance of phase 2 gene regulation in protection against electrophile and reactive oxygen toxicity and carcinogenesis[J].Adv Enzyme Regul, 2016, 43(1):121-134 [10]Stefanson A L, Bakovic M.Dietary Regulation of Keap1Nrf2ARE Pathway: Focus on Plant-Derived Compounds and Trace Minerals[J].Nutrients, 2014, 6(9):3777-3801 [11]Vasanthi H R, Mukherjee S, Das D K.Potential health benefits of broccoli- a chemico-biological overview[J].Mini Reviews in Medicinal Chemistry, 2009, 9(6):749- [12]Noh J R, Kim Y H, Hwang J H, et al.Sulforaphane protects against acetaminophen-induced hepatotoxicity[J][J]. Food & Chemical Toxicology, , 2015, 80:193-200. [13]Kensler T W, Egner P A, Agyeman A S, et al.Keap1–Nrf2 Signaling: A Target for Cancer Prevention by Sulforaphane[J].Topics in Current Chemistry, 2013, 329(8):163-177 [14]Hu R, Xu C, Shen G, et al.Gene expression profiles induced by cancer chemopreventive isothiocyanate sulforaphane in the liver of C57BL6J mice and C57BL6JNrf2 (--) mice[J].Cancer Letters, 2006, 243(2):170-192 [15]Fernandes R O, Bonetto J H, Baregzay B, et al.伪[J].Molecular & Cellular Biochemistry, 2014, 401(1-2):61-70 [16]Mi L, Xiao Z, Hood B L, et al.Covalent Binding to Tubulin by Isothiocyanates: A MECHANISM OF CELL GROWTH ARREST AND APOPTOSIS*S?[J].Journal of Biological Chemistry, 2008, 283(32):22136-22146 [17]Youn H S, Kim Y S, Park Z Y, et al.Sulforaphane suppresses oligomerization of TLR4 in a thiol-dependent manner[J].Journal of Immunology, 2010, 184(1):411-419 [18]Greaney A J, Maier N K, Leppla S H, et al.Sulforaphane inhibits multiple inflammasomes through an Nrf2‐independent mechanism[J].Journal of Leukocyte Biology, 2016, 99(1):189- [19]Kensler T W, Egner P A, Agyeman A S, et al.Keap1-Nrf2 Signaling: A Target for Cancer Prevention by Sulforaphane[J].Topics in Current Chemistry, 2013, 329(8):163-177 [20]Zhou C, Poulton E J, Grün F, et al.The dietary isothiocyanate sulforaphane is an antagonist of the human steroid and xenobiotic nuclear receptor[J].Molecular Pharmacology, 2007, 71(1):220- [21]Bryant C S, Kumar S, Chamala S, et al.Sulforaphane induces cell cycle arrest by protecting RB-E2F-1 complex in epithelial ovarian cancer cells[J].Molecular cancer, 2010, 9(1):47- [22]Chaudhuri D, Orsulic S, Ashok B T.Antiproliferative activity of sulforaphane in Akt-overexpressing ovarian cancer cells[J].Molecular Cancer Therapeutics, 2007, 6(1):334-345 [23]Ashok B T, Chaudhuri D.Anti-proliferative targets of sulforaphane in ovarian cancer[J].[J].Cancer Research,, 2005, 65:- [24]叶娜, 董晓英, 李冬华.卵巢早衰的颗粒细胞凋亡机制研究进展[J].首都医科大学学报, 2014, v.35(3):379-383 [25]Lim J, Ortiz L, Nakamura B N, et al.Effects of deletion of the transcription factor Nrf2 and benzo [a]pyrene treatment on ovarian follicles and ovarian surface epithelial cells in mice[J].Reproductive Toxicology, 2015, 58(10):24-32 [26]Mmh S, Konca Y, Akyuz B, et al.Concentration dependent antioxidative and apoptotic effects of sulforaphane on bovine granulosa cells in vitro[J]. , : [J].Theriogenology, 2017, 97:17-26. [27]Sohel M H, Cinar M U, Kalibar M, et al.Appropriate concentration of Hydrogen Peroxide and Sulforaphane for granulosa cells to study oxidative stress in vitro[J].[J].Journal of Biotechnology,, 2016, 231:S24-S24 [28]Chen H, Landen C N, Li Y, et al.Enhancement of Cisplatin-Mediated Apoptosis in Ovarian Cancer Cells through Potentiating G2M Arrest and p21 Upregulation by Combinatorial Epigallocatechin Gallate and Sulforaphane[J].Journal of Oncology, 2013, 2013(2013):872957- [29]Hunakova L, Gronesova P, Horvathova E, et al.Modulation of cisplatin sensitivity in human ovarian carcinoma A2780 and SKOV3 cell lines by sulforaphane[J].Toxicology Letters, 2014, 230(3):479-486 [30]Birner P, Schindl M, Obermair A, et al.Expression of hypoxia-inducible factor 1alpha in epithelial ovarian tumors: its impact on prognosis and on response to chemotherapy[J].Clinical Cancer Research, 2001, 7(6):1661-1668 [31]Pastorek M, Simko V, Takacova M, et al.Sulforaphane reduces molecular response to hypoxia in ovarian tumor cells independently of their resistance to chemotherapy[J].International Journal of Oncology, 2015, 47(1):51-60 [32]Hussain A, Priyani A, Sadrieh L, et al.Concurrent sulforaphane and eugenol induces differential effects on human cervical cancer cells[J].Integrative Cancer Therapies, 2011, 11(2):154- [33]Sharma C, Sadrieh L, Priyani A, et al.Anti-carcinogenic effects of sulforaphane in association with its apoptosis-inducing and anti-inflammatory properties in human cervical cancer cells[J].Cancer Epidemiology, 2011, 35(3):272-278 [34]Yang X M, Cai-Hong L I, Sun D X, et al.Study on the inhibitory effects and mechanism of Sulforaphane on human cervical cancer Hela cell[J]. [J].Chinese Journal of Biochemical & Pharmaceutics, 2016., :- [35]Cheng Y M, Ching-Chou T, Yi-Chiang H.Sulforaphane,a Dietary Isothiocyanate,Induces G2M Arrest in Cervical Cancer Cells through CyclinB1 Downregulation and GADD45βCDC2 Association[J].International Journal of Molecular Sciences, 2016, 17(9):1530-