Comprehensive Analysis of Prognostic Biomarkers in Cervical Squamous Carcinoma Based on Aberrant MiRNA-mRNA Axes and Cox Regression Models

doi:10.12280/gjfckx.20201204

Abstract

Abstract:

Objective: To explore the expression of microRNA(miRNA)-messenger RNA(mRNA) and predict its prognostic significance in cervical squamous carcinoma (CESC), analyze its significance in predicting the prognosis of CESC. Methods: TCGA database was used to screen the differential expression profiles of miRNA and mRNA in CESC and normal cervical tissue. The interaction between miRNA and targeted mRNA was verified by miRTarBase, TargetScan and miRDB databases. The prognostic markers of CESC were determined by Kaplan-Meier survival analysis and visualized by Cytoscape software. Construction the miRNA-mRNA regulatory network related to the prognosis of CESC, the selected mRNAs were analyzed by gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Results: Four miRNAs and nine mRNAs differentially expressed in CESC and normal tissues were detected. Four miRNA-mRNA axes closely related to the prognosis of CESC were obtained. The target mRNA of hsa-miR-505-5p was CBX8, and hsa-miR-142-3p targeted ADAMTS3, PTPRB and SEC23A respectively. Biological function and pathway analysis showed that the differentially expressed mRNAs were mainly enriched in biological process and PI3K-Akt pathway, HPV infection pathway. Conclusions: Above miRNA-mRNA axes obtained in this study are related to the prognosis of CESC, which can be used as a new perspective for the study of the pathogenesis and an effective indicator for monitoring the prognosis of CESC.

Key words: Uterine cervical neoplasms, The cancer genome atlas databases, Kaplan-Meier estimate, Prognosis, MicroRNAs, RNA,messenger

LI Yuan-xing, NIU Xiao-chen, CHANG Jing-jing, LUO Hai-xia, WANG Wei. Comprehensive Analysis of Prognostic Biomarkers in Cervical Squamous Carcinoma Based on Aberrant MiRNA-mRNA Axes and Cox Regression Models[J]. Journal of International Obstetrics and Gynecology, 2021, 48(5): 553-559.

Figures/Tables 6

References 22

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