Abstract: Objective: To study the expression of PKR, high risk HPV（HPV16/18） in cervical cancer and pre-malignant lesion, and discuss the significance and the possible mechanism in occurrence and development of cervical cancer and pre-malignant lesion by PKR. Methods: From February, 2006 to February, 2007, we took patients with cervical cancer（n=37） and patients with cervical intraepithelial neoplasia（CIN, n=56） treated in the department of gynecology in Tianjin first central hospital and Huanghe hospital, and compared with healthy donor （n=30）. In the 123 cases, we use immuno-histochemical SABC staining method to detect the expression of PKR and HPV16/18 E6 and analyze the happening and developing of cervical cancer and pre-malignant lesion and the effect in cervical lesion induced by PKR. Result: The positive rate of PKR expression in cervical cancer tissues was 54.1%. The positive rate of PKR expression in the cancer group is higher than CIN groups and the normal cervical group（P <0.05）. In the cancer group, the expression of PKR and HPV has inverse correlation （r =-0.554, P <0.05）, and in the CINⅢ group, the expression of PKR and HPV has inverse correlation（r =0.480, P =0.032）. Conclusion: PKR was strongly associated with a decreased risk of developing cervical cancer, and HPV E6 gene has relation to modulate PKR expression. The expression of PKR and E6 could be an indicator in the prognosis of cervical cancer.

Key words: Double-stranded RNA-dependent protein kinase, Human papilloma virus, Cervical intraepithelial neoplasia, Cervical cancer, Immunohistochemistry