Expression of SRGAP1 in Ovarian Cancer and Its Significance in Prognosis

doi:10.12280/gjfckx.20220152

Abstract

Abstract:

Objective: To investigate the clinical significance of the expression of Slit-Robo GTPase-activating protein 1 (SRGAP1) in ovarian cancer. Methods: The expression of SRGAP1 protein in benign ovarian cyst tissues, ovarian cancer tissues and peritoneal metastasis tissues was analyzed by immunohistochemistry. The results were verified by Western blotting and quantitative real-time PCR (qRT-PCR). The relationship between SRGAP1 and clinicopathological parameters (age, FIGO stage, histological type, pathological grade and lymph node metastasis) in patients with ovarian cancer was analyzed. Kaplan Meier plotter analyzed the correlation between SRGAP1 mRNA expression level and patient prognosis. Results: The expression of SRGAP1 in ovarian cancer tissues was higher than that in benign ovarian cyst tissues and peritoneal metastasis tissues (P<0.05). There were significant differences in histological types and pathological grades among high and low SRGAP1 expression(P<0.05). Compared with patients with low SRGAP1 expression, patients with high SRGAP1 expression had significantly shorter overall survival (OS) and progression-free survival (PFS), with statistically significant differences (P<0.001), while patients with high SRGAP1 expression had shorter progression-free survival (PPS), with no statistically significant difference (P=0.054). Conclusions: SRGAP1 expression is up-regulated in ovarian cancer, and its high expression is associated with poor prognosis of ovarian cancer, which can be considered as a specific marker of poor prognosis of ovarian cancer.

Key words: GTPase-activating proteins, Ovarian neoplasms, Ovarian cysts, Immunohistochemistry, Prognosis

XIE Wei-wei, WANG Xi-peng. Expression of SRGAP1 in Ovarian Cancer and Its Significance in Prognosis[J]. Journal of International Obstetrics and Gynecology, 2022, 49(3): 307-312.

Figures/Tables 4

References 23

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基因		序列
SRGAP1	上游	5′-ACAAGAAAGACCAGAACCTGTTG-3′
	下游	5′-TGCATGAACCGCATAATCACAT-3′
GAPDH	上游	5′-CTGGGCTACACTGAGCACC-3′
	下游	5′-AAGTGGTCGTTGAGGGCAATG-3′

基因		序列
SRGAP1	上游	5′-ACAAGAAAGACCAGAACCTGTTG-3′
	下游	5′-TGCATGAACCGCATAATCACAT-3′
GAPDH	上游	5′-CTGGGCTACACTGAGCACC-3′
	下游	5′-AAGTGGTCGTTGAGGGCAATG-3′

组别	n	年龄（岁）		FIGO分期		病理学分级		组织学分型		淋巴结转移
组别	n	<50 （n=43）	≥50 （n=73）	Ⅰ~Ⅱ （n=66）	Ⅲ~Ⅳ （n=50）	低级别（n=64）	高级别（n=52）	非上皮性（n=13）	上皮性（n=103）	阳性（n=38）	阴性（n=78）
SRGAP1低表达组	39	20（46.51）	19（26.03）	24（36.36）	15（30.00）	22（34.37）	17（32.69）	6（46.15）	33（32.04）	12（31.58）	27（34.62）
SRGAP1高表达组	77	23（53.49）	54（73.97）	42（63.64）	35（70.00）	42（65.63）	35（67.31）	7（53.85）	70（67.96）	26（68.42）	51（65.38）
χ²		0.662		0.948		8.867		6.432		0.353
P		0.444		0.208		0.005		0.021		0.733

组别	n	年龄（岁）		FIGO分期		病理学分级		组织学分型		淋巴结转移
组别	n	<50 （n=43）	≥50 （n=73）	Ⅰ~Ⅱ （n=66）	Ⅲ~Ⅳ （n=50）	低级别（n=64）	高级别（n=52）	非上皮性（n=13）	上皮性（n=103）	阳性（n=38）	阴性（n=78）
SRGAP1低表达组	39	20（46.51）	19（26.03）	24（36.36）	15（30.00）	22（34.37）	17（32.69）	6（46.15）	33（32.04）	12（31.58）	27（34.62）
SRGAP1高表达组	77	23（53.49）	54（73.97）	42（63.64）	35（70.00）	42（65.63）	35（67.31）	7（53.85）	70（67.96）	26（68.42）	51（65.38）
χ²		0.662		0.948		8.867		6.432		0.353
P		0.444		0.208		0.005		0.021		0.733