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    Gynecological Diseases & Related Research:Review
    The Role and Mechanism of Autophagy in Endometriosis
    LIU Ke-yi, LIU Heng-wei
    2022, 49 (3):  241-245.  doi: 10.12280/gjfckx.20211119
    Abstract ( 1672 )   HTML ( 84 )   PDF (769KB) ( 5924 )  

    Endometriosis (EMs) is a common benign gynecologic disease in women of childbearing age, which is difficult to be cured and complicated. Until now, the exact pathogenesis of EMs is not well known. Autophagy is a dynamic regulatory process in which biomembrance encapsulates and transports damaged organelles and misfolded proteins into the lysosomes for degradation and recycling. Recently an increasing number of studies proved that autophagy plays a vital role in the pathogenesis, diagnosis and treatment of EMs. The expression level of autophagy in EMs is up-regulated or down-regulated, suggesting that autophagy plays a double-edged sword role in promoting or inhibiting the occurrence and development of EMs, but many key problems remain unsolved. This article reviews the latest progress in the mechanism of autophagy in the pathogenesis and treatment of EMs.

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    Potential Role of Autophagy in the Pathogenesis of Chronic Endometritis
    WANG Xing, YANG Ru-yu, SITU Xiao-hua, LIANG Yan-chun
    2022, 49 (3):  246-249.  doi: 10.12280/gjfckx.20210984
    Abstract ( 1598 )   HTML ( 36 )   PDF (667KB) ( 4262 )  

    Chronic endometritis (CE) can lead to infertility, recurrent pregnancy loss and recurrent implantation failure. The specific pathogenesis mechanism of CE remains unknown. Abnormal autophagy was proved in the endometrium of patients with CE, indicating that autophagy plays an important role in the development of CE. Researches have confirmed that the development of CE is related with abnormal distribution and polarization of macrophages, as well as abnormal activation of NOD-like receptor protein 3 (NLRP3) inflammasome, both of which are modulated by autophagy. Thus, we propose that autophagy may activate NLRP3 inflammasome by regulating macrophage polarization, thereby leading to the occurrence of CE. This review illustrates a new insight for the pathogenesis of CE and provides new potential targets for the treatment of CE.

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    Risk and Clinical Characteristics of Thrombotic Disease in Patients with Adenomyosis
    ZHANG Hui-yuan, WANG An-qi, WANG Xia, ZHU Shu, WANG Xiu-li
    2022, 49 (3):  250-255.  doi: 10.12280/gjfckx.20211090
    Abstract ( 2082 )   HTML ( 46 )   PDF (695KB) ( 4265 )  

    Adenomyosis is one of the most common benign diseases in gynecology. The clinical manifestations of adenomyosis are dysmenorrhea, menorrhagia and infertility, which seriously affect the quality of life of patients. In recent years, domestic and foreign scholars have reported more than 30 cases of thrombotic disease in patients with adenomyosis, including cerebral infarction, disseminated intravascular coagulation and venous thromboembolism, most of which occurred during menstruation. After analyzing the characteristics of these patients, it was found that the risk factors for thrombotic disease in patients with adenomyosis included inflammation, increased tissue factor expression, increased blood CA125 level, menorrhagia and enlarged uterine. Once thrombotic diseases occur in women with adenomyosis, it will increase the difficulty of clinical diagnosis and treatment. Understanding the changes of coagulation function in patients with adenomyosis and being alert to the risk of thrombotic diseases will help us to improve the accuracy and safety for diagnosing and treating adenomyosis.

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    Predictors and Ovulation Induction Therapy for Polycystic Ovary Syndrome Patients with Clomiphene Resistance
    MA Li-na, MA Hong-li, LI Jian, CONG Jing, GAO Jing-shu, WANG Yu, WU Xiao-ke
    2022, 49 (3):  256-260.  doi: 10.12280/gjfckx.20211135
    Abstract ( 1676 )   HTML ( 26 )   PDF (696KB) ( 4214 )  

    Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility. Ovulation induction is the first choice of treatment for PCOS patients with fertility requirements. According to the current domestic and international treatment guidelines, clomiphene citrate (CC) is the traditional first-line drug to induce ovulation for infertile women with PCOS. However, the response of CC ovulation treatment varies significantly for different patients. It was reported that about 40% of PCOS patients cannot respond to CC, that is, CC resistance. This paper provides a review on the research progress of predictive indicators for PCOS infertile women with CC resistance as well as alternative ovulation induction strategies in recent years. This paper mainly expounds the predictive indicators for PCOS patients with CC resistance from the perspective of phenotype, biochemical characteristics and genetics. Single or combined schemes are selected in the treatment methods of letrozole (LE), gonadotropin (Gn), laparoscopic ovarian perforation (LOD), in vitro fertilization (IVF) and adjunctive therapy of traditional Chinese medicine to elaborate their alternative ovulation induction strategies, and the advantages and potential shortcomings of these single and combined schemes are discussed to provide more accurate and individualized ovulation induction schemes for PCOS infertile women.

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    Construction of Mouse Model of Polycystic Ovary Syndrome
    HUANG Qian-qian, WANG Jiao-jian, SONG Dian-rong
    2022, 49 (3):  261-266.  doi: 10.12280/gjfckx.20210970
    Abstract ( 1711 )   HTML ( 28 )   PDF (802KB) ( 4296 )  

    Polycystic ovary syndrome(PCOS) is considered to be one of the most common female endocrine diseases with complex etiology and high incidence rate. Its diverse clinical manifestations and long-term complications seriously affect the physical and mental health and quality of life of patients. Therefore, the establishment of a practical and effective experimental model is very important to the research of PCOS. Rats and mice are one of the most commonly used experimental animals because of their small size, short reproductive cycle and stable estrous cycle. This paper reviews the research progress of mouse models of PCOS at home and abroad based on the clinical characteristics, hormone levels, endocrine and metabolic indexes of PCOS, aims to evaluate the advantages and disadvantages of various models and provide guidance for the selection of models in experiments.

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    Research on Gynecological Malignancies:Review
    Clinical Analysis of 9 Adolescent Ovarian Endometriosis Recurrence Cases after Conservative Surgery
    SUN Lu, FAN Xiao-dong, LI Wen, YANG Yi-nan, ZHU Ying-jun
    2022, 49 (3):  267-271.  doi: 10.12280/gjfckx.20220251
    Abstract ( 1615 )   HTML ( 31 )   PDF (757KB) ( 4289 )  

    Objective: To explore the possible causes of postoperative recurrence in adolescent patients with ovarian endometriosis (EMs). Methods: Retrospectively analyzed the clinical data of 9 adolescent ovarian endometriosis recurrent cases who underwent conservative operation. Results: The clinical stage of 9 patients was stage Ⅰ in 1 case, stage Ⅲ in 1 case and stage Ⅳ in 7 cases. 7 patients had dysmenorrhea recurred 3 to 4 months after drug treatment completed. The ovarian EMs recurred in 7 patients within 1 year, 1 patient after 3 years, 1 patient after 4 years followed the first operation. 3 patients had lesion recurred in the contralateral ovary, 3 patients had lesion recurred in the ovary with tiny focus during the primary operation, 2 patients had lesion recurred at the same ovary and 1 patient had bilateral ovarian recurred. 5 patients had experienced recurrence once, 3 patients had recurred twice, and one patient had recurred three times after the primary surgery. 2 patients had delivered at full term after operation. 9 patients had long term drug management 2 to 6 medication cycles after primary surgery. Conclusions: The clinical stage of the primary surgery is usually severe in adolescent recurrence patients after conservative operation, dysmenorrhea and EMs lesion recurrence usually occur within 1 year after surgery. Severe clinical stage, dysmenorrhea, unsatisfied long term drug management and low willing to pregnancy may relate with recurrence after surgery.

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    Research Progress on Molecular Pathological Classification of Endometrial Cancer
    REN Wen-bin, CUI Xiang-rong, ZHANG San-yuan
    2022, 49 (3):  272-277.  doi: 10.12280/gjfckx.20210953
    Abstract ( 2859 )   HTML ( 36 )   PDF (714KB) ( 4554 )  

    Endometrial carcinoma is a malignant tumor with significant molecular and histological heterogeneity. Different pathological types have different biological behaviors and histological characteristics. Similar to advanced cancers of other histological subtypes, adjuvant radiation therapy is usually used for early-stage endometrioid carcinoma and chemotherapy is usually used for serous endometrial carcinoma. Therefore, the correct classification of subtypes is the key to choosing appropriate adjuvant therapy regimen. At present, the clinical classification of endometrial cancer still refers to Bokhman classification and WHO pathological classification. With the comprehensive promotion of precision medicine and molecular diagnosis and treatment technology, the limitations of traditional endometrial cancer typing methods in the individualized treatment of endometrial cancer, prognosis assessment and related genetic disease screening have become increasingly prominent. An optimized classification method is urgently needed in clinic to provide the exact theoretical and practical basis. In 2013, The Cancer Genome Atlas (TCGA) research center in the United States confirmed the molecular classification of endometrial cancer by integrating genomic features. Compared with Bokhman′s and WHO′s classification, this classification achieved a stronger correlation with the prognosis of patients with endometrial cancer, which opened the curtain for the molecular diagnosis and treatment of endometrial Cancer.

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    Research Progress on Molecular Characteristics and Targeted Therapy of Uterine Serous Carcinoma
    XIE Yun-kai, KONG Wei-min
    2022, 49 (3):  278-281.  doi: 10.12280/gjfckx.20210973
    Abstract ( 1913 )   HTML ( 24 )   PDF (718KB) ( 4384 )  

    As a special type of endometrial carcinoma, uterine serous carcinoma (USC) has high malignant degree, high risk of invasion and metastasis and poor prognosis. With the deepening of molecular research on endometrial carcinoma, molecular characteristics have been applied to the pathological diagnosis, treatment and prognosis of endometrial carcinoma. A variety of gene mutations have been found in USC, which may help to guide clinical diagnosis and therapy. And the specific molecular changes provide potential therapeutic targets for USC targeted therapy. At present, a variety of targeted therapies, including HER2 inhibitors, immune checkpoint inhibitors, anti-angiogenesis therapy, PI3K pathway inhibitors, PARP inhibitors and so on, have been used in the clinical treatment of the disease. Targeted therapy is expected to become a new breakthrough in the treatment of USC.

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    Advances of Robotic Systems in Comprehensive Staging Surgery for Early Ovarian Cancer
    FU Xiao-yu, GUO Rui-xia
    2022, 49 (3):  282-285.  doi: 10.12280/gjfckx.20210698
    Abstract ( 1743 )   HTML ( 18 )   PDF (737KB) ( 4313 )  

    Surgery is the main treatment for early ovarian cancer. Early diagnosis and standardized treatment can significantly prolong the 5-year survival rate of patients. Robot-assisted laparoscopic surgery breaks the limitations of traditional laparoscopic surgery, it has 3D stereoscopic vision, high-definition and stable lens, flexible inner wrist with 7 degrees of freedom, and improved ergonomics. It can perform fine operations in deep and narrow operating spaces and it also has the advantages of less intraoperative bleeding, less postoperative complications and faster recovery. So it has excellent results in complex operations compared with traditional laparoscopic surgery. In recent years, the research on robot-assisted laparoscopy in the comprehensive staging of early ovarian cancer has gradually increased, the development of minimally invasive surgery, especially the application of robotic surgery systems, provides a new direction for the treatment of early ovarian cancer, choosing an appropriate surgical method is of great significance for the treatment of early ovarian cancer. This article mainly reviews the adequacy, feasibility and safety of robotic systems in early ovarian cancer surgery.

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    LIU Yan, BIAN Wei, XIAO Hong
    LIU Yan, BIAN Wei, XIAO Hong
    2022, 49 (3):  286-290.  doi: 10.12280/gjfckx.20220089
    Abstract ( 1675 )   HTML ( 16 )   PDF (700KB) ( 4887 )  

    CyclinE1(CCNE1) gene amplification is one of the most common copy number variations in high-grade serous ovarian carcinoma (HGSOC), with an amplification rate of about 20%. CCNE1 gene amplification not only participates in the early precancerous lesions of HGSOC and promotes the occurrence and development of malignant tumors, but also causes cell cycle disorder and chromosome instability, thus increasing platinum resistance and recurrence rate of HGSOC, and ultimately leading to poor chemotherapy effect and poor prognosis of patients. In order to improve the prognosis and quality of life of patients with CCNE1-amplified HGSOC, cyclin-dependent kinases 2(CDK2) small molecule inhibitors or poly (ADP-ribose) polymerase (PARP) inhibitors in combination with other drugs are used to treat CCNE1-amplified HGSOC in the absence of targeted drugs for CCNE1-amplified HGSOC. To a certain extent, it can effectively inhibit the proliferation of tumor cells and promote the death of tumor cells. Therefore, CCNE1 gene is expected to become a potential therapeutic target and prognostic biological indicator of HGSOC from the perspective of the influence of CCNE1 amplification on the early pathogenesis of HGSOC and the generation of platinum resistance, which is of great significance to improve the treatment and prognosis of patients.

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    Research Progress of Histone Methyltransferase EZH2 in Cervical Cancer
    WANG Zhao-di, WANG Yue
    2022, 49 (3):  291-295.  doi: 10.12280/gjfckx.20210940
    Abstract ( 1551 )   HTML ( 16 )   PDF (706KB) ( 4296 )  

    Enhancer of zeste homolog 2 (EZH2) is a kind of histone lysine methyltransferase (HKMT). It participates in the malignant behavior of tumorigenesis and development mainly through regulating the methylation modification of H3K27, which plays an important role in epigenetic modification. The researches show that EZH2 is highly expressed in cervical cancer and it is closely related to the poor prognosis of patients. EZH2 takes part in the proliferation, migration, invasion, angiogenesis, drug resistance and immune escape of cervical cancer. The in-depth study of EZH2 is expected to provide a new therapeutic target for cervical cancer, so as to contribute to the early diagnosis, prognosis evaluation and targeted treatment of cervical cancer.

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    The Value of Imaging in the Diagnosis of Pelvic and Abdominal Lymph Node Metastasis in Cervical Cancer
    LIU Yi, LIU Qing, LIU Kai-jiang, CHENG Jie-jun
    2022, 49 (3):  296-301.  doi: 10.12280/gjfckx.20210895
    Abstract ( 1660 )   HTML ( 18 )   PDF (712KB) ( 4256 )  

    In 2018, the International Federation of Gynecology and Obstetrics (FIGO) updated cervical cancer staging, defining pre-treatment imaging diagnosis of pelvic lymph node metastasis as stage ⅢC1r and paraaortic lymph node metastasis as stage ⅢC2r. Preoperative imaging assessment of pelvic and abdominal lymph node metastasis is related to the formulation of treatment plans, and accurate imaging judgment of pelvic and abdominal lymph node status can clarify the stage and allow patients to receive standardized treatment. There are many imaging methods in clinical practice, including ultrasound, magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET)/CT and PET/MRI. Ultrasound is economical and convenient, but the detection rate of lymph nodes is too low. CT is widely used, with fast examination speed and large scanning range, which makes clinical application easy to promote, but the lack of uniform diagnostic criteria for CT diagnosis makes the diagnostic value not outstanding. MRI has good tissue resolution and can realize functional imaging at the same time, but the sensitivity of examination is not high. The accuracy of PET examination is higher than other imaging examinations, but its sensitivity is not statistically different from that of MRI and CT examination.

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    The Role of Metabolomics in Cervical Cancer Screening and Early Diagnosis
    GE Yan-jun, ZHANG Yu-chen, WANG Xin-tao, ZHU Hai-yan
    2022, 49 (3):  302-306.  doi: 10.12280/gjfckx.20210797
    Abstract ( 1610 )   HTML ( 22 )   PDF (719KB) ( 5001 )  

    Cervical cancer is the most common gynecological cancer, which is preventable and treatable. Improved screening and early detection played important role in reducing the incidence and mortality of cervical cancer. During the development of cervical cancer, there are often alterations in the type and quantity of metabolites, involving glucose metabolism, glutamine metabolism and lipid metabolism. Therefore, metabolomics has great potential in exploring mechanisms of cervical cancer initiation and development as well as new methods of screening and early detection. Through metabolite analysis of biological samples such as blood, urine, tissues and cells, we can find changes in metabolites closely related to cervical cancer and establish new screening and diagnostic methods with high sensitivity and specificity as well as convenience and good acceptability. The aim of this review is to explore the possible application of metabolomics in screening and early detection of cervical cancer.

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    Research on Gynecological Malignancies:Original article
    Expression of SRGAP1 in Ovarian Cancer and Its Significance in Prognosis
    XIE Wei-wei, WANG Xi-peng
    2022, 49 (3):  307-312.  doi: 10.12280/gjfckx.20220152
    Abstract ( 1356 )   HTML ( 43 )   PDF (2624KB) ( 4308 )  

    Objective: To investigate the clinical significance of the expression of Slit-Robo GTPase-activating protein 1 (SRGAP1) in ovarian cancer. Methods: The expression of SRGAP1 protein in benign ovarian cyst tissues, ovarian cancer tissues and peritoneal metastasis tissues was analyzed by immunohistochemistry. The results were verified by Western blotting and quantitative real-time PCR (qRT-PCR). The relationship between SRGAP1 and clinicopathological parameters (age, FIGO stage, histological type, pathological grade and lymph node metastasis) in patients with ovarian cancer was analyzed. Kaplan Meier plotter analyzed the correlation between SRGAP1 mRNA expression level and patient prognosis. Results: The expression of SRGAP1 in ovarian cancer tissues was higher than that in benign ovarian cyst tissues and peritoneal metastasis tissues (P<0.05). There were significant differences in histological types and pathological grades among high and low SRGAP1 expression(P<0.05). Compared with patients with low SRGAP1 expression, patients with high SRGAP1 expression had significantly shorter overall survival (OS) and progression-free survival (PFS), with statistically significant differences (P<0.001), while patients with high SRGAP1 expression had shorter progression-free survival (PPS), with no statistically significant difference (P=0.054). Conclusions: SRGAP1 expression is up-regulated in ovarian cancer, and its high expression is associated with poor prognosis of ovarian cancer, which can be considered as a specific marker of poor prognosis of ovarian cancer.

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    Research on Gynecological Malignancies:Case Report
    Well-Differentiated Papillary Mesothelioma Combined with Pelvic Endometriosis:A Case Report and Literature Review
    XU Qian, LIU Xin, WANG Jin-juan, XU Fei
    2022, 49 (3):  313-316.  doi: 10.12280/gjfckx.20210969
    Abstract ( 1572 )   HTML ( 40 )   PDF (5317KB) ( 4370 )  

    Well-differentiated papillary mesothelioma is a rare disease, and it is often found accidentally during surgery for other diseases. Its etiology is unknown, and its cause may be related to endometriosis. However, due to its low incidence, gynecologists generally do not know enough about it, which is easy to cause missed or misdiagnosed. This paper reported a case of well-differentiated papillary mesothelioma. The patient was admitted to the hospital for laparoscopic surgery for uterine fibroids. Intraoperative exploration revealed scattered patchy milky white corn-like lesions of 0.2-0.5 cm in diameter in the pelvic peritoneum and the posterior uterine wall serous membrane, with pelvic peritoneal endometriosis lesions next to them. Intraoperative biopsy of the lesion was performed, and well-differentiated papillary mesothelioma was diagnosed by histological pathology, immunohistochemistry and molecular detection. The pathogenesis, clinical manifestations, diagnosis, differential diagnosis and treatment of this disease were reviewed through the literature, and the diagnosis and treatment experience of this case was summarized.

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    Recurrence of Superficial Cervicovaginal Myofibroblastoma after Resection: A Case Report and Literature Review
    AN Jing, BI Xue-han, KANG He-yao, YANG Yong-xiu
    2022, 49 (3):  317-320.  doi: 10.12280/gjfckx.20211188
    Abstract ( 1963 )   HTML ( 20 )   PDF (4860KB) ( 4351 )  

    Superficial cervicovaginal myofibroblastoma (SCVM) is a newly recognized and relatively rare benign tumor with independent clinical and pathological features. It mostly occurs in postmenopausal women, mainly in the vagina, vulva and cervix. It is easy to be misdiagnosed and clinically. It can easily be confused with fibrous epithelial polyps, angiomyofibroblastoma and cellular angiofibroma. A clear diagnosis depends on pathological morphology, immunohistochemistry and clinical data. Some cases have a clear history of taking anti-estrogen drugs and may have relapse or recurrence. Now we retrospectively analyze the clinical data of a patient with SCVM recurrence in the First Hospital of Lanzhou University, and review the literature, discuss its differential diagnosis, in order to improve the understanding of the tumor.

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    Malignant Transformation of Giant Abdominal Wall Endometriosis: A Case Report and Literature Review
    SUN Guo-dong, JIANG Yi, YUAN Lin, ZHOU Shu-lin, CHENG Wen-jun
    2022, 49 (3):  321-324.  doi: 10.12280/gjfckx.20210944
    Abstract ( 1521 )   HTML ( 40 )   PDF (3485KB) ( 4237 )  

    Endometriosis is a common benign disease in women of childbearing age, which mostly occurs in the uterus and ovary. Abdominal wall endometriosis (AWE) refers to the ectopic endometrial lesions formed by the ectopic growth of endometrial tissue to the abdominal wall. Although endometriosis is considered as a benign disease, a small part of it is still transformed into malignancy. About 80% of endometriosis related malignancies are found in the ovary, while 20% are limited to extragonadal sites, and the malignant transformation AWE is rare. Now we report the malignant case of AWE with large tumor volume in our hospital, summarize the clinical characteristics and pathogenesis of AWE, so as to diagnose and treat the disease early and prolong the survival time.

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    Obstetric Physiology & Obstetric Disease:Review
    Epithelial-Mesenchymal Transition and Decidualization in Endometrial Receptivity
    JIN Jie, XIONG Wen-qian, LIU Yi
    2022, 49 (3):  325-339.  doi: 10.12280/gjfckx.20210881
    Abstract ( 1856 )   HTML ( 26 )   PDF (822KB) ( 4291 )  

    Implantation of the embryo is a key step in the process of conception which involves complex factors. Successful implantation entails a viable embryo to be implanted into an acceptable endometrium at an appropriate time, and a state of synchronous communication between the embryo and the endometrium. Errors in any part of the implantation process will affect the pregnancy rate, wherein decreased endometrial receptivity may be implicated as one of the major causes of infertility. During the early stage of implantation, endometrial epithelial cells undergo epithelial-mesenchymal transition to facilitate embryo adhesion, followed by decidualization of endometrial stromal cells to provide a suitable environment for embryo development. This suggests that epithelial-mesenchymal transition and decidualization is the fundamental condition for maintaining the receptive state of the endometrium, and an acceptable endometrium is the biological basis for embryo implantation and full development.

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    Obstetric Management of Pregnancy Complicated with Thalassemia
    LI Yang, YANG Hai-lan
    2022, 49 (3):  330-334.  doi: 10.12280/gjfckx.20211170
    Abstract ( 1686 )   HTML ( 18 )   PDF (684KB) ( 4254 )  

    Pregnancy with thalassemia is a single-genome inherited hemolytic disease occurring during pregnancy. Due to the imbalance in the composition of hemoglobin in the blood, the blood system of pregnant patients has changed, which may affect the maternal heart function, increase the risk of thrombosis, damage the immune system, and disrupt endocrine system. Previous studies have focused on the epidemiology and pathogenesis of pregnancy-associated thalassemia, but lacked systematic elaboration on pregnancy management, genetic counseling and prenatal diagnosis of complications. This study started from the types of lesions and pathogenesis, and deeply analyzes the unique complications of thalassemia during pregnancy and the corresponding treatment methods, in order to achieve better pregnancy outcomes and perinatal outcomes.

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    Research Progress of Iron Deficiency and Iron Deficiency Anemia in Pregnancy
    HUANG Kai-kun, LIU Rui-xia, YIN Cheng-hong
    2022, 49 (3):  335-339.  doi: 10.12280/gjfckx.20220008
    Abstract ( 1704 )   HTML ( 26 )   PDF (688KB) ( 4312 )  

    The role of iron in oxygen transport, electron transfer, and enzyme activity is essential for cell function. During pregnancy, with the increase in the blood volume of the mother and the growth and development of the fetus, the demand for iron increases sharply. When the body′s iron storage and intake cannot meet the body′s needs, it will lead to a relative or absolute lack of iron and cause iron deficiency anemia. Iron deficiency during pregnancy is the most common cause of anemia during pregnancy and is related to offspring′s cognition, autism, and mental disorders. Anemia during pregnancy increases the risk of infection during the puerperium, abruption of the placenta, death of pregnant women, gestational hypertension, cesarean delivery, postpartum hemorrhage, fetal growth restriction, and preterm birth, etc, it endangers the health and safety of mothers and babies, so early diagnosis and treatment are critical. Iron deficiency anemia in pregnancy is treated with oral iron and intravenous iron. This review summarized the diagnosis of iron deficiency and iron deficiency anemia during pregnancy, and discussed the adverse effects of iron deficiency anemia during pregnancy on pregnant women and their offspring, as well as its treatment and prevention strategies.

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    Obstetric Physiology & Obstetric Disease:Original Article
    Study on Z-Score Model of Normal Fetal Thymus
    GU Kai-li, FENG Tao, TIAN Li, DENG Miao-miao, WANG Li-qi
    2022, 49 (3):  340-344.  doi: 10.12280/gjfckx.20211093
    Abstract ( 1667 )   HTML ( 15 )   PDF (2467KB) ( 4260 )  

    Objective: To investigate the changes of fetal thymus transverse diameter, anterior and posterior diameter, area and circumference with gestational age (GA), and to establish the Z score model of normal fetal thymus from 20 to 38 weeks. Methods: From September 2020 to May 2021, 612 single pregnant women who underwent routine prenatal examinations in the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine were randomly selected. The transverse diameter, anterior and posterior diameter, area and circumference of the fetal thymus were measured. According to the correlation coefficients between the measured values and GA, biparietal diameter (BPD) and femur length (FL), the optimal indexes for evaluating fetal thymus size were selected. The optimal prediction regression equation and prediction standard deviation (SD) regression equation of fetal thymus were established based on GA, BPD and FL, and the Z-score model was constructed according to the formula Z value =(actual measured thymus value-predicted thymus value)/predicted SD. Results: The two-dimensional measurements of fetal thymus increased with the increase of GA, which was significantly positively correlated with GA (P<0.001). The correlation between thymus diameter and GA was the highest (r=0.873, P<0.001). The best fitting equations of fetal thymus diameter with GA, BPD and FL were linear. The predicted regression equation and the predicted standard deviation regression equation of fetal thymus diameter were established successfully, and the Z-values calculated by Shapiro-Wilk test satisfied normal distribution (P>0.05). Conclusions: Thymus transverse diameter is the best two-dimensional index to evaluate normal fetal thymus size, and the Z-score model of fetal thymus transverse diameter provides a basis for prenatal evaluation of fetal thymus development and early detection of diseases related to abnormal thymus development.

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    Prenatal Diagnosis and Genetic Counseling of Lesch-Nyhan Syndrome Family Caused by HPRT1 Gene Mutation
    GAO Shan-shan, DAI Peng, ZHAO Gan-ye, KONG Xiang-dong
    2022, 49 (3):  345-349.  doi: 10.12280/gjfckx.20210903
    Abstract ( 1823 )   HTML ( 14 )   PDF (2333KB) ( 4786 )  

    Objective: Prenatal diagnosis and genetic counseling of a Lesch-Nyhan syndrome family were performed by the studies of point mutation in the HPRT1 gene. Methods: The older child born by the patient was the Lesch-Nyhan Syndrome (proband),who was caused by the hemizygotic variation of HPRT1 gene (NM_000194) c.289_290delGT (p.V97Rfs *10). The second-born died, whose symptoms were similar to the older child, and HPRT1 gene test was not performed. Without pre-pregnancy genetic counseling before the third natural pregnancy, the method of polymerase chain reaction combined with Sanger sequencing was used to study the point mutation of HPRT1 gene in the family under the principle of informed consent, and genetic counseling was provided. Results: The hemizygotic variation c.289_290delGT(p.V97Rfs*10) of the HPRT1 gene (NM_000194) was detected in the proband. The heterozygous variation c.289_290delGT(p.V97Rfs*10) of the HPRT1 gene (NM_000194) was detected in the mother. The variation c.289_290delGT (p.V97Rfs*10) of the HPRT1 gene (NM_000194) was not detected in the fetus. The proband′s mother was advised to continue pregnancy, and finally gave birth to a healthy baby girl with normal physical examination at 1 year old. Conclusions: The mutation locus of HPRT1 gene of proband is inherited from the mother. Clinical attention should be paid to gene testing and prenatal diagnosis of other members of the proband′s lineage, and perfect genetic counseling should be given to avoid the birth of children with the Lesch-Nyhan syndrome and other rare genetic diseases.

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    The Therapy of Antenatal Hydronephrosis
    WU Wen-juan, LIU Kan, WANG Li, WANG Rui-li, WU Hai-ying
    2022, 49 (3):  350-353.  doi: 10.12280/gjfckx.20210988
    Abstract ( 1623 )   HTML ( 13 )   PDF (1149KB) ( 4316 )  

    Objective: To evaluate the perinatal consequence after fetal pelvic-amniotic shunt for bilateral antenatal hydronephrosis. Methods: A retrospective analysis of 35 fetuses with bilateral hydronephrosis was made in the Obstetrics Department of Henan Provincial People′s Hospital from January 2017 to October 2020 through multidisciplinary consultation. 7 cases in the intrauterine treatment group were given pelvic-amniotic shunt on the severe side of hydronephrosis, and 28 cases who had regular reexamination of the degree of hydronephrosis were conservative group, to analyze the relief of hydronephrosis and amniotic fluid volume after shunting, and the perinatal outcome, neonatal survival rate and operation rate of the two groups were compared. Results: Fetal hydronephrosis was relieved after shunt in 4 cases, accounting for 57.14% (4/7), and amniotic fluid volume improved in 6 cases, accounting for 85.71% (6/7). The compression of hydronephrosis on the fetal kidney parenchyma had remitted in different degrees after vesicoamniotic shunting, the 28-day survival rate of newborns and the operation rate in the intrauterine treatment group were significantly higher than that of the conservative group(P<0.05). There was no significant difference in the half-year survival rate and 1-year survival rate of the two groups(P>0.05). Conclusions: Pelvic-amniotic shunt can reduce the compression of hydronephrosis on the fetal kidney parenchyma, improve amniotic fluid volume, and can improve the 28-day survival rate of newborns.

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    Obstetric Physiology & Obstetric Disease:Case Report
    Umbilical Cord Knot in Monochorionic Monoamnionic: Two Cases Report
    CHENG Jun-xiu, WANG Qian-ting, DU Xiu-ping
    2022, 49 (3):  354-356.  doi: 10.12280/gjfckx.20211115
    Abstract ( 1881 )   HTML ( 23 )   PDF (3898KB) ( 4252 )  

    Monochorionic monoamnionic (MCMA) monozygotic twin is a very rare type of twin pregnancy, which is extremely high-risk twin pregnancy. Because the two fetuses share the same amniotic cavity and there is no fetal membrane separation between the two fetuses, there is a greater possibility of intrauterine accidents due to umbilical cord entanglement and knot. For pregnant women with twin pregnancies should be clear as soon as possible its chorionic properties. Once diagnosed as MCMA twins, vigilance should be raised and termination of pregnancy in the guidelines recommend gestational weeks to improve maternal and infant outcomes. This paper retrospectively reports two cases of monochorionic monoamnionic twins pregnancy with true umbilical knot and successful delivery in Shanxi Maternal and Child Health Hospital, to improve obstetricians′ awareness of early diagnosis and differentiation of twin chorionic properties. Special attention should be paid to the abnormality of umbilical cord morphology and structure when diagnosed as monochorionic monoamnionic twins, prenatal management of monochorionic monoamnionic twins pregnancies should be strengthened.

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    A Case Report of Neonatal Death Due to Fetal Arrhythmia
    GE Wen-qi, MA Qian, MI Yang
    2022, 49 (3):  357-360.  doi: 10.12280/gjfckx.20210906
    Abstract ( 1329 )   HTML ( 30 )   PDF (3347KB) ( 4244 )  

    Most fetal arrhythmias are benign processes in cardiac embryo development, transient and good prognosis. And persistent fetal arrhythmia or arrhythmia with heart structural defect, fetal edema, can cause perinatal adverse outcome. In this case, persistent supraventricular arrhythmia with fetal edema was treated with digoxin monotherapy, the pregnancy terminated by caesarean section at 36 weeks of gestation and the newborn died 3 days after birth. By reviewing the patient′s general situation and diagnosis and treatment process, summarizing experience and lessons, and strengthening clinicians′ understanding of the disease and its treatment plan, for patients with fetal arrhythmia, perinatal maternal and child monitoring should be strengthened, and individualized treatment plans should be formulated to improve adverse pregnancy outcome.

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