Abstract: Hyperandrogenism is one of the common features of polycystic ovarian syndrome（PCOS）. It is very important to discover and treat this disease early because of its high prevalent rate in the reproductive age women. Nowadays，the hospitals in all over the world still use ELISA to detect the Free testosterone and chemiluminescence to detect the Total testosterone. There are many studies about the assay methods and projects for androgen and most studies demonstrated that these assay methods for testosterone have been fraught with accuracy problems；they are not sensitive for the females because of their low serum androgen concentration. Meanwhile，the androgen determination projects still has not reached the consistent mutual recognition. It prompts there is possiblily a majority patients accompanied by hyperandrogenism have not been diagnosed. Radioimmunoassay analysis and the mass spectrometrybased methods are considered to be the highly effective，but it is very difficult to use in the clinic and diagnose the disease because of many reasons.

Key words: Polycystic ovary syndrome, Hyperandrogenism, Radioimmunoassay, Chromatography, high pressure liquid