Abstract: Toluidine blue（TB）is a vital dye used for nucleic acid staining. We can use the staining for detection of the early malignant or premalignant endometrium lesions. Since the 1980s，TB staining has been used in the investigation of oral，vulvar，and upper gastrointestinal tract malignancies. With the development of science and technology，advantages of TB staining in endometrial pathological diagnosis has gradually aroused great attention from domestic and foreign experts，however，due to the limit of techniques，equipment and many other factors，the research of the TB staining in endometrial cancer and endometrial precancer is still at its initial stage.Based on the above，this paper reviews the research progress of toluidine blue staining in the diagnosis of endometrial carcinoma and precancerous lesions.

Key words: Staining and labeling, Endometrial neoplasms, Precancerous conditions, Hysteroscopes, Diagnosis