Abstract: Objective： An HPLC method was established for determination of paclitaxel′s concentration in ovarian neoplasm patients′ plasma. Methods：Each test 500 μL plasma sample was alkalinized by sodium bicarbonate firstly，and then extracted by diethyl ether. Docetaxel was used as the internal standard. Analytical column was Ultimate Acclaim?誖120 C18（5 μm， 4.6 mm×250 mm）. The isocratic mobile phase was the acetonitrile and 0.1% phosphoric acid with the rate of 55∶45（V/V）. The flow rate was 1.3 mL/min， the temperature of chromatographic column was 20 ℃ and the detective UV wavelength was 227 nm. Results：The calibration curve was linear over the concentration range of 0.1-10.0 mg/L（r=0.998 9）. The limit of detection was 0.04 mg/L（S/N=3）， the relative recovery was（98.5±4.1）% and the RSD of intra-day and inter-day were lower than 10% respectively. Conclusions： The method is simple, rapid and repeatable. It is suitable for chemotherapeutic drug monitoring and the pharmacokinetics studying in ovarian neoplasm patients.

Key words: Ovarian neoplasms, Paclitaxel, Chromatography, high pressure liquid, Plasma concentration