Study on Effect of S1P/S1PR on Angiogenesis in SKOV3 Ovarian Cancer Cells

Abstract

Abstract: Objective：The study was to explore the effect and mechanism of S1P/S1PR on angiogenesis in human ovarian cancer cell line (SKOV3). Methods：Tube formation assay was to explore the angiogenetic effect of S1P on ovarian cancer cells. QRT-PCR was used to identify IL-8, IL-6 and VEGF mRNA expression changes in SKOV3 ovarian cancer cells incubation with S1P. SKOV3 cells were transfected with siRNA interference sequences silencing S1PR1, S1PR2 and S1PR3 gene. S1PR mRNA levels were determined by qRT-PCR and protein levels were determined by western-blot. IL-8, IL-6 and VEGF change levels in S1PR gene silencing ovarian cancer cell lines were detected by qRT-PCR. Results：The tube formation essay of human umbilical vein endothelial cells was significantly increased by the culture supernatants of ovarian cancer cells treated with S1P (t=-3.667, P=0.021). The results showed that S1P promoted the pro-angiogenic ability of SKOV3. The mRNA expression of IL-8, IL-6 and VEGF were significantly increased by S1P in SKOV3 cells (P＜0.05). The mRNA expression levels of IL-8, IL-6 and VEGF in SKOV3 cells were significantly decreased in S1PR1 and S1PR3 gene silencing cells (all P＜0.05) and were not significantly changed in S1PR2 gene silencing cells. Conclusions：The study indicated that S1P could affect ovarian cancer cells through S1PR1/3 to promote ovarian cancer angiogenesis and IL-8, IL-6, VEGF may play a role in this process. S1P/S1PR pathway is expected to become a new target for ovarian cancer therapy.

Key words: Ovarian neoplasms, Angiogenesis, Sphingosine-1-phosphate, Sphingosine-1-phosphate receptor

LIU Yi-xuan，DAI Lan，XIE Lei，GAO Hua，DI Wen. Study on Effect of S1P/S1PR on Angiogenesis in SKOV3 Ovarian Cancer Cells[J]. Journal of International Obstetrics and Gynecology, 2018, 45(3): 337-341.

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