Abstract: Objective：To detect the expression of mRNA and protein of the active ATP-binding cassette family G2 transporters (ABCG2) and the level of β-hCG before and after the RNA interference of ABCG2 gene in primary cultured trophoblast cells from normal and pre-eclamptic placentas, explore the effect of ABCG2 on the function of trophoblasts in pre-eclampsia. Methods：Three target siRNA sites of ABCG2 genes were determined by siRNA prediction tools and three pairs of complementary shRNA sequence transformation plasmids were synthesized. The effective interfering plasmids were selected to package lentivirus and infected trophoblasts. The change of expression of ABCG2 mRNA and protein in the cells were detected by real time fluorescence quantitative PCR and Western blotting in cultivated villous trophoblasts of different differentiation states from normal and pre-eclamptic placentas to verify the effect of interference, β-hCG level was detected by ELISA before and after interference in cell culture supernatants. Results：After RNA interference, the expression of ABCG2 mRNA and protein decreased significantly in trophoblasts and hCG secretion of trophoblasts was reduced. The level of hCG in pre-eclampsia trophoblasts was higher than normal before and after interference. Conclusions：SiRNA could effectively interfere the expression of ABCG2 gene in trophoblast cells. Secretion of hCG was inhibited after the silence of ABCG2 gene expression in placental trophoblasts, hCG secretion in pre-eclampsia patient was higher than normal. ABCG2 might play a role in inhibiting the development of pre-eclampsia by stimulating the secretion of hCG of trophoblasts.

Key words: Pre-eclampsia；, Trophoblasts；, RNA, small interfering；, Chorionic gonadotropin, beta subunit, human；, ABCG2