Clinical Application of Stem Cell-Derived Exosomes in Gynecological Diseases

doi:10.12280/gjfckx.20230955

Abstract

Abstract:

Stem cell-derived exosomes (SC-Exo) are petite vesicles, released from stem cells. Exosomes originating from different cells or tissues play diverse roles in the pathogenesis and progression of diseases, and have made significant achievements and progress in various clinical fields. The application value of SC-Exo in gynecological diseases such as recurrent spontaneous abortion, polycystic ovary syndrome, premature ovarian insufficiency, intrauterine adhesions, wound repair in vaginal surgery, gynecological tumors and pelvic floor dysfunction is increasingly being recognized. SC-Exo can mediate the signaling and functional transfer of genetic information between targeted cells by releasing a variety of bioactive molecules such as nucleic acids, proteins, lipids and cytokines. It has potential benefits for the treatment of gynecological diseases and is a novel and promising therapeutic strategy.

Key words: Stem cells, Exosomes, Genital diseases, female, Mesenchymal stem cells, MicroRNAs

GUO Shi-wei, SUN Jing-li, CHEN Zhen-yu, LIU Sen. Clinical Application of Stem Cell-Derived Exosomes in Gynecological Diseases[J]. Journal of International Obstetrics and Gynecology, 2024, 51(3): 279-283.

References 28

[1]	岂航, 许天敏. 间充质干细胞治疗妇科系统疾病的临床研究进展[J]. 中国计划生育和妇产科, 2022, 14(10):30-34. doi: 10.3969/j.issn.1674-4020.2022.10.08.
[2]	路春华, 盛修贵. 妇科肿瘤相关间充质干细胞研究进展[J]. 中华肿瘤防治杂志, 2015, 22(12):989-992. doi:10.16073/j.cnki.cjcpt.2015.12.019.
[3]	叶青松, 彭友俭, 骆瑜. 细胞外泌体的分离提取标准化及临床转化进展[J]. 口腔疾病防治, 2022, 30(9):609-619. doi: 10.12016/j.issn.2096-1456.2022.09.001.
[4]	Huang B, Lu J, Ding C, et al. Exosomes derived from human adipose mesenchymal stem cells improve ovary function of premature ovarian insufficiency by targeting SMAD[J]. Stem Cell Res Ther, 2018, 9(1):216. doi: 10.1186/s13287-018-0953-7. pmid: 30092819
[5]	贾岳, 李妍, 王英骥. 外泌体靶向递送核酸类分子研究进展[J]. 中国医药导报, 2023, 20(3):33-36,49. doi: 10.20047/j.issn1673-7210.2023.03.07.
[6]	Shen Y, TanTai J. Exosomes secreted by metastatic cancer cells promotes epithelial mesenchymal transition in small cell lung carcinoma: The key role of Src/TGF-β1 axis[J]. Gene, 2024,892:147873. doi: 10.1016/j.gene.2023.147873.
[7]	郭玲慧, 王炜, 蒋露, 等. 外泌体膜蛋白功能研究进展[J]. 世界科学技术-中医药现代化, 2020, 22(10):3622-3628. doi: 10.11842/wst.20191217004.
[8]	相燕洁. 间充质干细胞来源的外泌体对流产小鼠母-胎免疫耐受的调节[D]. 济南: 山东大学, 2021.
[9]	Cao M, Zhao Y, Chen T, et al. Adipose mesenchymal stem cell-derived exosomal microRNAs ameliorate polycystic ovary syndrome by protecting against metabolic disturbances[J]. Biomaterials, 2022, 288:121739. doi: 10.1016/j.biomaterials.2022.121739.
[10]	Zhao Y, Pan S, Wu X. Human umbilical cord mesenchymal stem cell-derived exosomes inhibit ovarian granulosa cells inflammatory response through inhibition of NF-κB signaling in polycystic ovary syndrome[J]. J Reprod Immunol, 2022,152:103638. doi: 10.1016/j.jri.2022.103638.
[11]	Zhao Y, Tao M, Wei M, et al. Mesenchymal stem cells derived exosomal miR-323-3p promotes proliferation and inhibits apoptosis of cumulus cells in polycystic ovary syndrome (PCOS)[J]. Artif Cells Nanomed Biotechnol, 2019, 47(1):3804-3813. doi: 10.1080/21691401.2019.1669619. pmid: 31549864
[12]	Pu X, Zhang L, Zhang P, et al. Human UC-MSC-derived exosomes facilitate ovarian renovation in rats with chemotherapy-induced premature ovarian insufficiency[J]. Front Endocrinol(Lausanne), 2023,14:1205901. doi: 10.3389/fendo.2023.1205901.
[13]	Li Z, Zhang M, Zheng J, et al. Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Improve Ovarian Function and Proliferation of Premature Ovarian Insufficiency by Regulating the Hippo Signaling Pathway[J]. Front Endocrinol(Lausanne), 2021, 12:711902. doi: 10.3389/fendo.2021.711902.
[14]	Gao T, Chen Y, Hu M, et al. MicroRNA-22-3p in human umbilical cord mesenchymal stem cell-secreted exosomes inhibits granulosa cell apoptosis by targeting KLF6 and ATF4-ATF3-CHOP pathway in POF mice[J]. Apoptosis, 2023, 28(7/8):997-1011. doi: 10.1007/s10495-023-01833-5.
[15]	Xiao B, Zhu Y, Huang J, et al. Exosomal transfer of bone marrow mesenchymal stem cell-derived miR-340 attenuates endometrial fibrosis[J]. Biol Open, 2019, 8(5):bio039958. doi: 10.1242/bio.039958.
[16]	Yuan D, Guo T, Qian H, et al. Exosomal miR-543 derived from umbilical cord mesenchymal stem cells ameliorates endometrial fibrosis in intrauterine adhesion via downregulating N-cadherin[J]. Placenta, 2023, 131:75-81. doi: 10.1016/j.placenta.2022.11.013.
[17]	Saribas GS, Ozogul C, Tiryaki M, et al. Effects of uterus derived mesenchymal stem cells and their exosomes on asherman′s syndrome[J]. Acta Histochem, 2020, 122(1):151465. doi: 10.1016/j.acthis.2019.151465.
[18]	Zhao S, Qi W, Zheng J, et al. Exosomes Derived from Adipose Mesenchymal Stem Cells Restore Functional Endometrium in a Rat Model of Intrauterine Adhesions[J]. Reprod Sci, 2020, 27(6):1266-1275. doi: 10.1007/s43032-019-00112-6. pmid: 31933162
[19]	范琳媛, 王静璇, 魏薇, 等. 人脐带间充质干细胞来源的外泌体对盆底脱垂患者阴道壁成纤维细胞的调节作用[J]. 现代妇产科进展, 2022, 31(11):813-819. doi: 10.13283/j.cnki.xdfckjz.2022.11.031.
[20]	Zhu Z, Zhang Y, Zhang Y, et al. Exosomes derived from human umbilical cord mesenchymal stem cells accelerate growth of VK2 vaginal epithelial cells through MicroRNAs in vitro[J]. Hum Reprod, 2019, 34(2):248-260. doi: 10.1093/humrep/dey344.
[21]	Abas BI, Demirbolat GM, Cevik O. Wharton jelly-derived mesenchymal stem cell exosomes induce apoptosis and suppress EMT signaling in cervical cancer cells as an effective drug carrier system of paclitaxel[J]. PLoS One, 2022, 17(9):e0274607. doi: 10.1371/journal.pone.0274607.
[22]	Suzuki H, Yokoi A, Uno K, et al. Small Extracellular Vesicles from adipose-derived stem cells suppress cell proliferation by delivering the let-7 family of microRNAs in ovarian cancer[J]. Biochem Biophys Res Commun, 2023, 680:211-219. doi: 10.1016/j.bbrc.2023.09.022.
[23]	Xiao Y, Peng Y, Zhang C, et al. hucMSC-derived exosomes protect ovarian reserve and restore ovarian function in cisplatin treated mice[J]. J Biomed Res, 2022, 37(5):382-393. doi: 10.7555/JBR.36.20220166.
[24]	Pan Y, Wang X, Li Y, et al. Human umbilical cord blood mesenchymal stem cells-derived exosomal microRNA-503-3p inhibits progression of human endometrial cancer cells through downregulating MEST[J]. Cancer Gene Ther, 2022, 29(8/9):1130-1139. doi: 10.1038/s41417-021-00416-3.
[25]	Xu L, Sima Y, Xiao C, et al. Exosomes derived from mesenchymal stromal cells: a promising treatment for pelvic floor dysfunction[J]. Hum Cell, 2023, 36(3):937-949. doi: 10.1007/s13577-023-00887-6.
[26]	Liu X, Wang S, Wu S, et al. Exosomes secreted by adipose-derived mesenchymal stem cells regulate typeⅠcollagen metabolism in fibroblasts from women with stress urinary incontinence[J]. Stem Cell Res Ther, 2018, 9(1):159. doi: 10.1186/s13287-018-0899-9.
[27]	Ni J, Li H, Zhou Y, et al. Therapeutic Potential of Human Adipose-Derived Stem Cell Exosomes in Stress Urinary Incontinence-An in Vitro and in Vivo Study[J]. Cell Physiol Biochem, 2018, 48(4):1710-1722. doi: 10.1159/000492298.
[28]	Wu R, Huang C, Wu Q, et al. Exosomes secreted by urine-derived stem cells improve stress urinary incontinence by promoting repair of pubococcygeus muscle injury in rats[J]. Stem Cell Res Ther, 2019, 10(1):80. doi: 10.1186/s13287-019-1182-4. pmid: 30849996