Comparison of the Application Value of HPV E6/E7 mRNA and HPV DNA Detection in the Diagnosis and Treatment of Cervical Lesions

doi:10.12280/gjfckx.20201209

Abstract

Abstract:

Objective: To compare the application value of high risk human papillomavirus (HPV) E6/E7 mRNA and HPV DNA detection in the diagnosis and treatment of cervical cancer. Methods: From January 2018 to September 2019, a total of 33 496 patients received cervical liquid-based thin-prep cytology test (TCT) in Shi Jia Zhuang Maternal & Child Healthcare Hospital. Among them, 3 190 patients who met inclusion criteria (ASCUS) were randomly divided into control group and observation group, with 1 595 cases in each group. Control group received HPV DNA test and the other group received HPV E6/E7mRNA test. Then, cervical biopsy under colposcopy and histopathological examination were performed for positive patients in each group. The detection rate of cervical lesions was compared between the two methods. Then, 184 patients were randomly selected from the control group with positive result to receive HPV E6/E7 mRNA test. The other 184 patients were randomly selected from the observation group with positive result for HPV DNA detection. The diagnostic efficacy of the two methods was compared in 368 cases. Results: The detection rate of high-grade Cervical intraepithetial neoplasia was 77/1 595 (4.8%) in the control group and 66/1 595 (4.1%) in the observation group, with no statistically significant difference ( χ ²=0.886,P=0.347). However, compared with HPV DNA test, HPV E6/E7 mRNA test had higher sensitivity (87.50%), specificity (71.79%), positive predictive value (49.36%), negative predictive value (94.81%) and lower colposcopy referral rate (42.4%). The difference was statistically significant (P<0.05). Conclusions: The diagnostic value of HPV E6/E7 mRNA test is higher than that of HPV DNA test for high-grade cervical lesions. It could be used as a new method to triage patients with ASCUS.

Key words: Uterine cervical diseases, Human papillomavirus DNA tests, DNA, RNA,messenger, Papillomaviridae, Diagnosis, HPV E6/E7 mRNA test

LIU Xiao-xu, LIANG Jian-mei, SU Xue-yan, WANG Su-juan, ZHAO Hui-juan, QIN Li-xin, LU Xue-jing, WANG Ya-fan. Comparison of the Application Value of HPV E6/E7 mRNA and HPV DNA Detection in the Diagnosis and Treatment of Cervical Lesions[J]. Journal of International Obstetrics and Gynecology, 2021, 48(4): 462-466.

Figures/Tables 5

References 25

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年龄	例	构成比（%）
20~29岁	646	20.25
30~39岁	999	31.32
40~49岁	865	27.12
50~59岁	510	15.99
60~65岁	170	5.32
合计	3 190	100.00

年龄	例	构成比（%）
20~29岁	646	20.25
30~39岁	999	31.32
40~49岁	865	27.12
50~59岁	510	15.99
60~65岁	170	5.32
合计	3 190	100.00

组别	n	检测结果阳性	病理检查结果
组别	n	检测结果阳性	宫颈炎	CINⅠ	CINⅡ	CINⅢ	宫颈癌
对照组	1 595	417	144	196	33	38	6
观察组	1 595	402	152	184	27	35	4

组别	n	检测结果阳性	病理检查结果
组别	n	检测结果阳性	宫颈炎	CINⅠ	CINⅡ	CINⅢ	宫颈癌
对照组	1 595	417	144	196	33	38	6
观察组	1 595	402	152	184	27	35	4

组别	n	<CINⅡ	≥CINⅡ
HPV18阳性	44	42（95.5）	2（4.5）
HPV16阳性	205	152（74.1）	53（25.9）
其他HPV亚型阳性	168	146（86.9）	22（13.1）
χ²		16.313
P		0.000