Abstract:

In recent years, assisted reproductive technology (ART) has been increasingly applied in clinical practice. However, some patients still experience repeated implantation failure (RIF). A key underlying factor is impaired endometrial receptivity. Current clinical methods for assessing endometrial receptivity have limitations. Metabolomics, with its ability to dynamically and systematically reveal metabolic network changes in the endometrium during the implantation window, offers a new perspective for endometrial receptivity evaluation. Metabolomics studies based on different biological samples such as endometrial fluid, endometrial tissue, vaginal microbiota, and peripheral blood have demonstrated the value and limitations of identifying local or systemic metabolic biomarkers. These studies indicate that disturbances in key pathways, including amino acid, lipid, and energy metabolism, are common features in RIF patients. This review summarizes advances in the application of metabolomics techniques to screen for endometrial receptivity-related metabolic biomarkers in RIF populations by analyzing samples from different sources, such as endometrial fluid, endometrial tissue, and peripheral blood, aiming to provide references for individualized diagnosis and treatment strategies for RIF.

Key words: Repeated implantation failure, Endometrial receptivity, Metabolomics, Biomarkers, Implantation window