Abstract: Pre-eclampsia (PE) is a disease special to pregnancy. Its occurrence and development is an important reason for the increase of maternal and infant morbidity and mortality. Normal proliferation, migration and differentiation of trophoblasts in placenta are closely related to it. P27 acts as a cyclin dependent kinase inhibitors (CKIs), which regulates cell cycle by binding to cell cycle factors. Compared with normal pregnant women, the expression of P27 gene in placental trophoblasts of PE patients increased significantly, which may be related to the dysfunction of trophoblast proliferation and invasion. Nodal, as a member of transforming growth factor β (TGF-β), up-regulates the level of P27 mRNA and protein in trophoblast cells through various mechanisms, improves the stability of P27 protein and induces phosphorylation of P27 protein at S10, promotes the transfer of P27 protein and CDK2 to cytoplasm, and enhances the binding of P27/CDK2/CDK5/Stathmin, thus inhibiting cell proliferation and migration，transfer and invasion. P27 protein is phosphorylated at many siｔｅｓ, oxidative stress, protein kinase B (Akt), protein O-fucosyltransferase 1 (poFUT1), fructose-2-kinase/fructose-2, 6-diphosphatase (PFKFB1-4) affect the ubiquitin-proteasome degradation pathway by phosphorylating P27 protein.

Key words: Pre-eclampsia, Placenta, P27 protein, Cell cycle, Cell proliferation