The Effect of BCL6 on Proliferation, Apoptosis, Invasion, and Migration of HTR-8/SVneo Trophoblast Cells

doi:10.12280/gjfckx.20240555

Abstract

Abstract:

Objective: To explore the effects of overexpression of B cell lymphoma 6 (BCL6) gene on the proliferation, apoptosis, invasion, and migration of human trophoblast cells HTR-8/SVneo. Methods: Using lentivirus mediated overexpression of LV5-BCL6 (OE-BCL6) technology to construct HTR-8/SVneo cell lines with BCL6 overexpression, while a negative control group (OE-NC) was set up. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the mRNA and protein expression levels of BCL6. Subsequently, CCK8 assay, flow cytometry, Transwell assay, and Transwell chamber invasion assay were performed to compare the proliferation, apoptosis, invasion, and migration abilities of two groups of HTR-8/SVneo cells. Results: Fluorescence microscopy observation showed that the slow virus transfection of HTR-8/SVneo cells in the OE-BCL6 group and OE-NC group resulted in good cell growth and infection efficiency of over 70%, indicating successful slow virus transfection. mRNA level detection showed that BCL6 expression in the OE-BCL6 group was higher than that in the OE-NC group, and the difference was statistically significant (P<0.001). Protein level detection showed that the expression of BCL6 in the OE-BCL6 group was higher than that in the OE-NC group, and the difference was statistically significant (P<0.001). After transfection for 48 and 72 hours, the proliferation activity of OE-BCL6 group cells decreased compared to OE-NC group, while the apoptosis rate of OE-BCL6 group cells increased compared to OE-NC group, and the differences were statistically significant (all P<0.05). The amount of cells invaded and migratory cells in the OE-BCL6 group was significantly lower than that in the OE-NC group (both P<0.05). Conclusions: The BCL6 gene can inhibit the proliferation, invasion, and migration of HTR-8/SVneo cells, and promote cell apoptosis. BCL6 may be a potential target for diagnosis and treatment of clinical pathological pregnancy, but further research is needed to elucidate its molecular mechanism.

Key words: B cell lymphoma 6, Human trophoblast cells HTR-8/SVneo, Cell proliferation, Apoptosis, Invasion

YANG Li, YANG Jing, YE Erdengqieqieke, HAN Rui, LA Xiao-lin. The Effect of BCL6 on Proliferation, Apoptosis, Invasion, and Migration of HTR-8/SVneo Trophoblast Cells[J]. Journal of International Obstetrics and Gynecology, 2024, 51(6): 659-663.

Figures/Tables 4

References 18

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组别	n	0 h	24 h	48 h	72 h
OE-NC组	3	100.00±1.46	100.01±7.91	100.00±2.68	100.00±1.37
OE-BCL6组	3	99.65±0.71	100.39±1.29	74.25±2.91	77.05±3.99
t		0.378	-0.083	11.287	9.417
P		0.725	0.938	<0.001	<0.001

组别	n	0 h	24 h	48 h	72 h
OE-NC组	3	100.00±1.46	100.01±7.91	100.00±2.68	100.00±1.37
OE-BCL6组	3	99.65±0.71	100.39±1.29	74.25±2.91	77.05±3.99
t		0.378	-0.083	11.287	9.417
P		0.725	0.938	<0.001	<0.001