Abstract: Objective：To make a retrospective analysis about 1 506 prenatal diagnosis cases of α-thalassemia and 665 cases of β-thalassemia in the prenatal diagnosis center of women and children hospital of Guangdong Province. Methods：Gap-PCR and polymerase chain reaction-reverse dot blot（PCR-RDB）assay. DNA sequencing was used in the case when unusual mutation was identified in at least one parent. Results：262 cases of Bart′s fetuses and 72 cases of Hb H were detected in 1 506 cases of α-thalassemia prenatal diagnosis. Meanwhile，174 fetuses with complex mutation were final diagnosed from 665 cases of β-thalassemia prenatal diagnosis. Conclusions：Prenatal diagnosis of thalassemia can effectively reduce the birth of Bart′s fetuses and seriously β-thalassemia，and it has the vital significance for eugenic birth and decrease of the perinatal complication.

Key words: Alpha-thalassemia, Beta-thalassemia, Prenatal diagnosis, Genes, DNA mutational analysis, Hydrops fetalis