Abstract: Objective：To analyze the effect of continuous stretch on oxytocin receptors and prostaglandin receptors expression of uterine smooth muscle cells under high glucose circumstance. Methods：Rat uterine smooth muscle cells were separated and cultured under high glucose circumstance for different durations, then we detected oxytocin receptors and prostaglandin receptors expression changes of smooth muscle cells. Continuous stretch was forced on cells under high glucose circumstance to determine synergistic effect of stretch and high glucose. At the same time, as a control team, advanced glycation end products (AGEs) inhibitor (sRAGE) was added to antagonize the effect of high glucose, then evaluate the expression of oxytocin receptors and prostaglandin receptors. Results：Both high glucose circumstance and continuous stretch could lead to oxytocin receptors and prostaglandin receptors expression of rat uterine smooth muscle cells increasing. Exerting high glucose and continuous stretch simultaneously could promote oxytocin receptors and prostaglandin receptors expressions even further and synergistic effect could be detected. After adding sRAGE, oxytocin receptors and prostaglandin receptors expressions decreased to some extend, but not completely, still higher than the normal glucose teams. Conclusions：Both continuous stretch and high glucose could promote oxytocin receptors and prostaglandin receptors expressions and there exist a synergistic effect between them. AGEs was involved in this process, but this is not the only signal pathway.

Key words: Stress, mechanical, Glucose, Uterus, Myocytes, smooth muscle, Receptors, oxytocin, Receptors, prostaglandin