Table of Content

15 April 2016, Volume 43 Issue 2
Previous Issue    Next Issue
---
综述
论著
综述
论著
综述
论著


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

综述

Select

Research Progress of Laminin 332 in Gynecological Tumors XIE Wei-min;WU Yi-lin 2016, 43 (2):  125-127.  Abstract ( 1260 )   PDF (496KB) ( 5838 )   Laminin 332 is a major component of the basement membrane in epithelial tissues and plays roles in the adhesion, migration and differentiation of epithelial cells. Laminin 332 consists of three different gene products, α3, β3 and γ2 chains, resulting in a heterotrimeric glycoprotein. Laminin 332 is synthesized by epithelial cells as a precursor. After secretion into the extracellular matrix, the α3 and γ2 chains undergo specific proteolytic processing to produce a mature form. Under the physiological condition, the key function of laminin 332 is sustaining the stable adhesion of epithelial cells to the underlying basement membrane in epithelial tissues. Laminin 332 has been shown to be highly expressed in various types of human malignant tumors and correlate well with tumor occurrence and development. The article is intended to review the structure of laminin 332, its relationship with malignant tumors and research progresses with gynecological tumors. Related Articles | Metrics

Select

Research Progress of miR-155 in Gynecological Malignant Tumor GAO Ya-nan;FENG Yu-zhen 2016, 43 (2):  128-130.  Abstract ( 1221 )   PDF (353KB) ( 5811 )   MicroRNAs (miRNAs) are small endogenous non-coding RNA molecules capable of regulating gene expression in posttranscriptional level, miRNAs are involved in a variety of biological processes such as cell cycle regulation, differentiation, growth, metabolism and aging. miR-155 is one of the most studied miRNA, which is expressed in many human cancers. Recent studies have shown that the expression profiling of miR-155 shows evidently abnormal in ovarian cancer, cervical cancer and endometrial cancer which indicated that miR-155 might play an important role in regulate the occurrence and development of gynecological tumors. Here is to summarize the expression of miR-155 in ovarian cancer, cervical cancer and endometrial cancer and its significance in the early detection, clinical diagnosis, treatment and prognosis of tumour, as well as their research status in gynecologic malignant tumor. Related Articles | Metrics

Select

Antidepressant Treatment Application Research Progress in the Gynecology Malignant Tumor RAN Xing-xing;QIU Li-hua 2016, 43 (2):  131-134.  Abstract ( 1026 )   PDF (476KB) ( 5865 )   Clinical found gynecological malignant tumor patients often appear different degree of psychological barriers, including depression which is one of the common negative emotions in gynecologic malignant tumor patients. Different levels of depression and other negative emotions have certain adverse impact on the immunity, chemical treatment and quality of life in patients with malignant tumor, which can further affect their therapeutic effect and prognosis. Psychological intervention, antidepressant drug and other antidepressive treatment in patients with gynecological malignant tumor has irreplaceable position in the process of comprehensive treatment. We review the research progress of antidepressant treatment applied in the gynecology malignant tumor, and mainly focuses on the influence of depression in patients with gynecological tumors and the main antidepressant treatment. Related Articles | Metrics

Select

Bevacizumab for Advanced Gynecological Cancer DU Wen-jing;DONG Xin-fang;LIU Da-jiang;YANG Yong-xiu 2016, 43 (2):  135-139.  Abstract ( 1287 )   PDF (481KB) ( 5879 )   As clinical researches for malignant tumors developing, molecular targeted therapy, following radical surgery, chemotherapy and radiotherapy, has become a novel and the 4th treatment for the advanced gynecological cancers. It also gathered more and more attention of cancer researchers due to its high level of specificity and abilities of enhance the effect of the anticancer, extension the survival period of patient. More and more attention has been attracted by molecular targeted therapy of anti VEGF antibody, and bevacizumab becomes the focus of attention. Bevacizumab is a monoclonal antibody that can specifically combine with VEGF, which is the key regulatory factor on the growth of tumor blood vessels. By the process mentioned above, bevacizumab can prevent VEGF from combining with its receptor, thus inhibit the formation of tumor blood vessels and achieve anticancer efficacy. At present, bevacizumab is acting as first-line medicine to treat ovarian cancer, recurrent or advanced metastatic cervical cancer, colorectal cancer, lung cancer and renal cell carcinoma which was approved by the Food and Drug Administration (FDA). Recently, treating advanced gynecological cancers with the use of bevacizumab, which is regarded as a novel therapeutic, becomes the focus of numerous studies. This paper reviews research progress in bevacizumab to treat advanced gynecological cancers. Related Articles | Metrics

Select

Updates of Patient Derived Xenografts Model in Ovarian Tumors WU Jian-fa;YI Xiao-fang 2016, 43 (2):  140-144.  Abstract ( 1043 )   PDF (501KB) ( 5898 )   Ovarian cancer has the highest motality among the female reproductive malignancies. The underling mechanism is still unknown, and the prognosis has not been improved significantly. To establish an ideal preclinical model is in favor of the study of recurrence and metastasis in ovarian cancer. Recently, the development of patient derived xenografts model gives us a new platform for preclinical study. Gradually it has been used in the study of breast cancer, lung cancer and colon cancer, where it is proved to be useful. Much less studies on the use of patient derived xenografts model in ovarian cancer has been reported and data was shown promising. Based on the existing literature retrieval, this model could better copy the biological characteristics of the primary tumor and keep the heterogeneity of primary tumor. But there were still some problems need to be solved in the application of it, such as the different tumor take rate, the limited tumor resources and the lack of effective quality control standards. Here this review aims to address the application status, existing problems and application prospects of this model. Related Articles | Metrics

Select

Molecular Mechanisms of Cisplatin and Paclitaxel Resistance for Ovarian Cancer LI Shu-qing;CHEN Ya-ping 2016, 43 (2):  145-150.  Abstract ( 1264 )   PDF (577KB) ( 5847 )   Ovarian cancer is the most lethal in female reproductive carcinomas, seriously threatening the lives and health of female. Cytoreductive surgery and platinum-based cytotoxic chemotherapy are standard therapies for the treatment of ovarian cancer. The majority of ovarian cancer patients are diagnosed at advanced stages due to insidious onset, while advanced ovarian cancer patients tend to become refractory to current chemotherapeutic agents. Therefore, a critical contributor to the poor survival rate is the development of chemoresistance. There is particularly significant implication to elucidate the molecular mechanisms of chemoresistance for exploring efficient targeted therapies and developing novel therapeutic agents that can improve chemotherapy sensitivity and reverse chemoresistance. The development of chemoresistance for ovarian cancer is the results of polygenic and multifactorial interactions at different levels, the significant mechanisms involve in anti-apoptosis, pro-survival, drug efflux, detoxification, DNA repair and so on. The molecular mechanisms of ovarian cancer resistance for cisplatin and paclitaxel are uncertain, now this report aims to review the possible mechanisms. Related Articles | Metrics

Select

The Detection and Significance of Pelvic Lymph Node Status in Diagnosis and Treatment of Cervical Cancer and Endometrial Carcinoma DENG Li-hui;FU Chun 2016, 43 (2):  151-155.  Abstract ( 1274 )   PDF (587KB) ( 5857 )   Lymph node metastasis as the main route of metastasis of cervical cancer and endometrial cancer, and is an important factor affecting the prognosis of cervical cancer and endometrial cancer. The way how to detect and evaluate the lymph node status before and during the therapy has still been in the preliminary exploration. Current methods of detecting lymph node status are ultrasonography, computer tomography, magnetic resonance imaging (MRI), positron emission tomography/computer tomography (PET-CT) and localization by sentinel lymph node biopsy etc. Different methods create images in different ways and have different sensitivity, specificity and accuracy. With the development of radiological technology and the imaging agents, the detection levels of lymph node status have been developed from simple morphology to function. Color Doppler ultrasonography, diffusion-weighted imaging, PET-CT. etc are more sensitive and specific when compared with traditional methods. Among them, PET-CT and functional MRI imaging have advantages in the detection of sensitivity, specificity and accuracy. Imaging agents are subdivided into three kinds of agents detecting tumor cell metabolism, vessel and lymphatic drainage through development. Related Articles | Metrics

Select

Research Status of Long-term Quality of Life in Cervical Cancer Survivors and Related Countermeasure GUAN Huai;HAN Lu;SHANG Li-xin 2016, 43 (2):  156-160.  Abstract ( 1020 )   PDF (590KB) ( 5766 )   Cervical cancer is the fourth most common cancer among women worldwide and is the main burden of gynaecologic cancer. Cases of cervical cancer in developing countries account for 85% of the global total, the situation is grim. In recent years, there continue to be improvements in its therapeutic method and treatment effectiveness. However, treatment-related effects, including urinary, gastrointestinal, sexual, lymphedema and psychological side effects, can disrupt long-term quality of life (QoL) in survivors. Factors influencing the above adverse outcomes mainly include treatment methods, surgical procedures and human factors. Factors that affect the poor prognosis include therapeutic method, surgical operation and cultural factors, etc. Methods improving QoL mainly include pharmacological treatment and surgical therapy. Certainly, improving therapeutic method and surgical procedures seems to be more important. Meanwhile, gaining support from family and society is helpful for the survivor. Related Articles | Metrics

Select

Relationship between Antiapoptotic Related Protein XIAP and Platinum Resistance of Ovarian Cancer YU Jin-yu;SHI Xiao-rong;LI Juan 2016, 43 (2):  161-164.  Abstract ( 1130 )   PDF (617KB) ( 5836 )   Tumor cell resistance to chemotherapeutic drugs has become more and more concerned in the clinical treatment, the tumor cells to escape apoptotic mechanisms become focusing points about the ongoing research in the resistance mechanism, XIAP is one of the chain of anti-apoptotic pathway of apoptosis protein family IAPs, respectively, play a key role in the development of kidney cancer, liver cancer, colon cancer, lung cancer, pancreatic cancer, ovarian cancer and other human tumor cell resistance, and studies have shown that high expression of XIAP in ovarian resistance cytoplasm of cancer cells can affect the growth of tumor cells through a variety of ways, but over the whole of the mechanism and countermeasures is not yet clear, now summarizes the current research progress about new progresses of XIAP gene are reviewed for the clinical treatment ovarian cancer drug resistance studies，and also provide new ideas. Related Articles | Metrics

论著

Select

Clinical Value of Human Epididymal Protein 4 in the Diagnosis of Endometrial Carcinoma in Atypical Endometrial Hyperplasia Patients DING Wei;LI Ying;ZHU Ying-jun 2016, 43 (2):  165-168.  Abstract ( 1281 )   PDF (633KB) ( 5831 )   Objective：To explore the clinical value of HE4 detection in the screening of endometrial carcinoma (EC) in atypical endometrial hyperplasia (AEH) patients diagnosed with curettage pathology. Methods：Between January 2011 and December 2014, the blood specimens were collected from the patients subjected to total hysterectomy with a preoperative biopsy diagnosis of AEH in Tianjin Central Hospital of Gynecology Obstetrics. Serum levels of HE4, CA125 and CA199 were detected by the electro-chemiluminescent immunoassay. According to the postoperative pathology, patients were divided into EC group and AEH group, and the differences of the tumor markers in the two groups were analyzed. Evaluate the diagnosis accuracy of the tumor markers by the area under the curve of receiver operating characteristic (ROC-AUC). Results：31 cases were diagnosed with postoperative EC in all the 118 preoperative atypical endometrial hyperplasia patients. Their serum level of HE4, CA125 and CA199 were 73.4 pmol/L, 31.4 kU/L and 23.3 kU/L. 87 were still AEH after the operation, the serum level of above tumor marker were 44.3 pmol/L, 17.0 kU/L and 19.0 kU/L. The median levels of HE4, CA125 in EC group were significantly higher than those in AEH group, the difference was statistically significant (P＜0.05), while the CA199 level in the two groups was not statistically different (P＞0.05). The ROC-AUC of HE4 and CA125 were 0.785 and 0.706 respectively. The AUC and sensitivity of joint detection were 0.867 and 76.6%. Conclusions：Serum HE4 and CA125 levels in EC group were significantly higher than those in AEH group. Combined detection of HE4 and CA125 is helpful to discriminate EC in patients with a preoperative diagnosis of AEH. Related Articles | Metrics

Select

A Meta-analysis of DNA-Methylated Gene APC and Ovarian Cancer YAN Bing-bing;LI Li 2016, 43 (2):  169-173.  Abstract ( 1218 )   PDF (686KB) ( 5791 )   Objective：To investigate the role of APC gene inactivation in the occurrence and development of ovarian cancer. Methods：Databases such as PubMed, EMBase, Web of Science, and CNKI data were searched. Related literatures were published from January 1, 2000 to December 31, 2014. Only randomized controlled trials were included. The quality of the included studies were assessed by Newcastle-Ottawa Scale(NOS). Stata 11.0 software was used for statistical analysis such as OR value，heterogeneity and publication bias. Results：Nine studies, with 641 ovarian cancer patients and 377 controls were included in the Meta-analysis. Statistical results shows that the pooled odds ratio was 6.19 (95%CI: 4.08-9.41, P=0.000) in the case group versus the control group under fix effects model. The pooled odds ratio was 5.88 (95%CI: 3.662-9.450, P=0.000) in the cancer group versus the normal group under fix effects model. The pooled odds ratio was 6.99 (95%CI: 3.12-15.64, P=0.000) in the cancer group versus the benign tumor group under fix effects model. Conclusions：This analysis suggests that APC gene methylation had an association with ovarian cancer. Related Articles | Metrics

Select

The Prognosis of Different Treatment for Stage ⅡB Cervical Cancer：A Meta-analysis CHEN Dan-dan;YANG Zhi-jun;LI Li 2016, 43 (2):  174-180.  Abstract ( 1045 )   PDF (857KB) ( 5861 )   Objective：To evaluate the effectiveness and safety of different treatments in stage ⅡB cervical cancer. Methods：Databases including PubMed, EMBase, Medline, The Cochrane Library（Issue 12, 2014）, CBM, CNKI, VIP and WanFang Data were search. According to the inclusion and exclusion criteria, two reviewers identified literature, extracted data and assessed quality independently. Then meta-analysis was performed using RevMan 5.3 software. Results：Three RCTs, one prospective trials, seventeen case-control studies involving 2 486 patients were included. NACT group compared with RS group, survival has not yet found statistically significant difference, the intraoperative blood loss, operation time, lymph node metastasis rate and postoperative complications of the former is lower than the latter. The 5-years OS and the incidencerate of radioactive cystitis are not statistical differences between multimodality therapy of surgery and concurrent chemoradiation. The incidence rate of radioactive cystitis of the former is less than the latter. The multimodality therapy of surgery compared with radiotherapy, there are no statistical differences in 3-year OS and the rate of fistula formation, but the 5-year OS of the former higher than the latter, the rate of radioactive enteritis of the former is lower than the latter. The gastrointestinal reaction′s rate and hematological toxicity′s rate are lower in the radiotherapy alone group than the concurrent chemoradiation group. Conclusions：The multimodality therapy of surgery treatment of stage ⅡB cervical cancer is the most effective treatment. Compared with RS, itcould reduce the intraoperative blood loss, operation time, lymph node metastasis rate and postoperative complications. Adverse reaction rate is lower than radiotherapy or concurrent chemoradiation. Therefore the multimodality therapy of surgery is more beneficial to improve the quality of life after treatment. Related Articles | Metrics

Select

Analysis of High Risk Factors of Early-stage Cervical Cancer with Pelvic Lymph Node Metastasis CHEN Qi;ZHANG Hong 2016, 43 (2):  181-183.  Abstract ( 1202 )   PDF (697KB) ( 5801 )   Objective：A statistical analysis was performed of the clinicopathology of stages Ⅰa2 and Ⅰb1 cervical cancer to analyse high risk factors of pelvic lymph node metastasis. Methods：A retrospective analysis was conducted of clinical data of a total of 117 patients with stage Ⅰa2 or Ⅰb1 cervical cancer who were hospitalized in Tianjin Central Hospital of Gynecology Obstetrics from January 2011 to June 2013. Use the χ2 test and logistic regression to analyse the high risk factors of pelvic lymph node. Results：Of the 117 patients, there were 15 (12.8%) and 102 (87.2%) with and without lymphatic metastasis, respectively. Findings of univariate analysis showed that depth of myometrial invasion ≥1/2, lower uterine segment involvement, positive intravascular tumor thrombosis, tumor size ＞2 cm and level of SCC-Ag before treatment were influencing factors for early-stage lymphatic metastasis of cervical cancer (all P＜0.05). Findings of multivariate analysis showed that SCC-Ag≥1.5 ng/mL was the independent influencing factor for lymphatic metastasis (OR=25.007, 95%CI: 2.342～250.021, P=0.008). Conclusions：Level of SCC-Ag before treatment was the high risk factor for pelvic lymph node metastasis of stages Ⅰa2 and Ⅰb1 cervical cancer. Related Articles | Metrics

Select

Effects of Phytic Acid Ketone on the Cell Proliferation of HeLa and Caski GAO Xiao-li;WANG Kan;TIAN Wen-yan;YUE Tian-fu 2016, 43 (2):  184-186.  Abstract ( 1254 )   PDF (671KB) ( 5863 )   Objective：To investigate the proliferation inhibitory effects of different density Phytic Acid Ketone on cervical cancer cell HeLa and Caski. Methods：Use the culture medium to dilute the type II No.1 and No.2 Phytic Acid Ketone to different density. The HeLa (adeno carcinoma, HPV18 genome integrated) and Caski (squamous carcinoma, HPV16 genome integrated) cells were treated with different concentration and time of Mefordcare. The inhibitory effects were evaluated with MTT assay. Results：The growth of HeLa and Caski was inhibited by different concentration of Mefordcare Phytic Acid Ketone, which was concentration-time dependent. The inhibitory effect on HeLa and Caski of Phytic Acid Ketone No. 1 and No. 2 varied with the concentration used, and the inhibitory rate came to the peak when concentration was about 5 860 mg/L and 5 500 mg/L. The inhibitory rate of cell proliferation was significantly different with the treating time at the same concentration (P＜0.05). Conclusions：There were inhibitory effects from Phytic Acid Ketone on the growth of cervical cancer cell line HeLa and Caski. The result provides theoretical basis for the clinical application of Mefordcare Phytic Acid Ketone. Related Articles | Metrics

Select

Comparison of Results between Dilatation and Curettage Pathology and Pos-tsurgical Gross Pathology in the Patients of Endometrial Cancer ZHANG Ying-lan;LYU Chang-shuai;ZHOU Xing-nan;LANG Jing-he 2016, 43 (2):  187-189.  Abstract ( 2306 )   PDF (650KB) ( 5842 )   Objective：To compare the diagnostic accuracy between dilatation and curettage (D&C) pathology and post-surgical gross pathology results in the patients of endometrial carcinoma. Methods：217 clinical data of patients who were diagnosed as endometrial carcinoma both by D&C and post-surgical gross pathology were collected, compared by the type of organization and histological grade to evaluate the accuracy of D&C pathology. Results：There were 196 endometrioid and 21 non-endometrioid carcinoma of the D&C pathology respectively, the sensitivity 97.4% and specificity 72.7%, Kappa value was 0.716 compared with the post-surgical gross pathology. The numbers of patients with grade 1, grade 2 and grade 3 were 135, 50 and 32 by D&C pathology. Compared with the post-surgical gross pathology, the weighted Kappa value was 0.596(Z=11.72，P=0.000). The misdiagnosis rate by D&C was significantly higher in grade 3 versus grade 1 and it was not significant in grade 2 versus the other two groups. Conclusions：Pre-surgical D&C pathology is very helpful and important in the pre-surgical evaluation of endometrial carcinoma, type of organization reveals higher consistency and grade 3 has much higher misdiagnosis rate compared with the grade 1 groups by D&C. Related Articles | Metrics

Select

Atypical Polypoid Adenomyoma of the Uterus: A Clinical Analysis of 43 Cases LI Wen-min;MA Bao-rong;DU Xiao-qin;PANG Shu-jie;LANG Yu-ping 2016, 43 (2):  190-192.  Abstract ( 1536 )   PDF (662KB) ( 6674 )   Objective：To discuss the clinical characteristics, diagnosis and treatment of atypical polypoid adenomyma (APA) of the uterus. Methods：A retrospective study was made of 43 patients with APA, who were pathologically confirmed in Tianjin Central Hospital of Gynecology Obstetrics between 2012-2013. Results：The age of these 43 patients ranged from 17-74, with an average of (47.0±2.3) years. 36 cases (83.72%) had irregular uterus bleeding clinically; 3 cases without symptoms indicated that there were intrauterine light in the body examination. 4 cases were found in the ovarian tumor surgery. 39 patients were performed hysteroscope, among which 28 cases were characteristically polypoid and 11 cases were multipolypoid. 28 cases were located in the uterine cavity. 4 cases were located in the lower uterine segment. 7 cases were located in the cervix. The diameter of polypoid were 1-4 cm. All of the cases were diagnosed by pathology. 34 cases of patients in the resection of lesions under hysteroscopy. 27 cases were successfully operated. 1 case gave up treatment. 15 patients had hysterectomy, among them, 5 patients were deteriorated with carcinoma of endometrium. Conclusions：Although most cases of APA were benign, a few cases were associated with low malignant potential of recurrence. The treatment should depend on the age and epithelial dysplasia degree and reproductive desire of patient, and long-term follow-up is suggested. Related Articles | Metrics

综述

Select

Potential Role of HMGB1 in Angiogenesis of Endometriosis WU Jin-jie;LIANG Yan-chun;YAO Shu-zhong 2016, 43 (2):  194-198.  Abstract ( 1135 )   PDF (771KB) ( 5791 )   Angiogenesis is one of the important pathogenesis of endometriosis. Abnormal neovascularization in endometriosis promotes the proliferation, infiltration and migration of ectopic endometrial cells. High mobility group box 1 protein (HMGB1), as an important pro-inflammatory factor, is recently proved to contribute to angiogenesis in different tumors. HMGB1 can promote neoangiogenesis by acting directly on endothelial cells or indirect effects on endothelial cells by vascular endothelial growth factor (VEGF), which is synthesized and secreted by NF-κB-activating macrophages. In addition, HMGB1 also promotes proliferation and migration of endothelial cells by up-regulating the expression of fibroblast growth factor (FGF) and enhancing the activity as well as the affinity of integrins, promotes the secretion of platelet-derived growth factor (PDGF) and then recruits pericytes and smooth muscle cells, activating the formation of vascular tubular structure. Activates the secretion of TGF-β from endothelial cells, finally promoting the maturation of vascular network. Here we review the potential mechanism of HMGB1 underlying the influence of pro-angiogenesis in endometriosis in detail, thus providing a new therapeutic target for endometriosis. Related Articles | Metrics

Select

Progress of Magnetic Resonance Imaging in Female Pelvic Organ Prolapse JIA Xiang;WU Qing-kai 2016, 43 (2):  199-202.  Abstract ( 1188 )   PDF (734KB) ( 5813 )   Pelvic floor dysfunction (PFD) is a kind of disease which is caused by the defects of the pelvic floor support structure. Pelvic organ prolapsed (POP) is an important part of PFD, the main clinical manifestations is the position of pelvic organs descent and the function abnormal, it can seriously affects the physical and mental health of adult women. It is necessary to study the mechanism of POP in female pelvic floor, which under the neural control, attached to the bony pelvis. It is a complex three-dimensional arrangement of muscle connective tissue and organs. Scholars use all kinds of physical examination and auxiliary examination to explore the anatomy and function of the pelvic floor, such as POP-Q score, ultrasound, defecography and magnetic resonance imaging (MRI). In recent years, the dynamic and static magnetic resonance imaging has the advantages of high resolution of flab, no radiation, multi planar imaging, no bone artifact interference etc, and has wide application prospect in the three-dimensional reconstruction of pelvic floor. Compare the advantages and disadvantages of magnetic resonance imaging with other methods in female pelvic floor imaging. Review the testing methods, application status and development prospects of magnetic resonance imaging in studying female pelvic floor diseases. Related Articles | Metrics

论著

Select

Different Expression and Clinical Significance of DNA Methyltransferases in the Chorionic Villi of Early Embryo Growth Arrest GUO Wei-wei;GAO Jing;ZHOU Lei;HUANG Wei-qing;KONG Qing-nuan 2016, 43 (2):  203-206.  Abstract ( 1095 )   PDF (834KB) ( 5862 )   Objective：To study the expression of DNMT1, DNMT3A, DNMT3B and DNMT3L mRNA and protein in the chorionic villi of early embryo growth arrest and explore its clinical significance. Methods：We randomly selected 40 women as observational group in which were diagnosed with early embryo growth arrest by B ultrasound and accepted complete curettage of uterine cavity after visiting the Obstetrical Department of the Qingdao Municipal Hospital between January 2013 and June 2014, during the same period, select another 40 women who performed induced abortion with normal early pregnancy as control group. ①Real-time quantitative PCR (qRT-PCR) was used on the normal groups (40 cases) and the early embryo growth arrest group (40 cases) to quantitatively determine DNMT1, DNMT3A, DNMT3B and DNMT3L mRNA expression in the chorionic villi tissues. ②Streptavidin-Perosidase (SP) immunochemistry and Western blot were performed on the two groups to detect the expression and distribution of DNMT1, DNMT3A, DNMT3B and DNMT3L. Results：①qRT-PCR showed there were no statistically significant difference of DNMT1, DNMT3A, DNMT3B and DNMT3L expression in two groups (P＞0.05). ②Immuno- chemistry showed DNMTs were predominately distributed on the villous trophoblasts which the cytoplasm and nuclear had varying degrees of positive staining. And semi-quantitative analysis indicated that the expression of DNMT3A of early embryo growth arrest was significantly lower, compared with that of the normal group (P＜0.05). ③Western blot also showed expression of DNMT3A of early embryo growth arrest was lower, differences between the two groups were statistical significance (P＜0.05). ④There was no significant correlation between DNMT3A and DNMT1, DNMT3B, DNMT3L in the chorionic villi of early embryo growth arrest (all P＞0.05). Conclusions：The lower expression of the DNMT3A protein might be involved in the pathogenesis of early embryo growth arrest. Related Articles | Metrics

Select

Obstetrical Outcome Analysis of Hysteroscopic Treatment of Uterus Septum WANG He;YANG Qing;BI Fang-fang 2016, 43 (2):  207-209.  Abstract ( 1151 )   PDF (780KB) ( 5794 )   Objective：To investigate the reproductive outcome after hysteroscopic resection of uterine septum in women with septate uterus. Methods：Clinical data of 231 patients who have received hysteroscopic septum resection in our hospital, follow-up of these patients with postoperative and reproductive outcome. Results：All patients complete the operation under laparoscopy or ultrasound surveillance, and no complications such as perforation or bleeding occurred. No obvious changes in menstruation and no intrauterine adhesions occurred at 6-30 months follow-up. 161 patients were pregnant at 2-25 months after the surgery, the pregnancy rate was 69.7% (161/231). 21 patients had spontaneous abortion, the abortion rate was 13% (21/161). 14 patients had preterm birth because of premature rupture of membrane, preterm birth rate was 10% (14/140). The pregnancy rate of different age groups were compared, and it was higher than that before operation, and it was more significant in the low age group. Conclusions：In hysteroscopic resection of uterrus septum can effectively improve the obstetrical outcome, improve the pregnancy rate and the live birth rate, and reduce the abortion rate. Patients who had found uterrus septum should be treated as early as possible to improve the pregnancy rate. Related Articles | Metrics

Select

Biological Effects of Ectopic Endometrium after Treatment on the Endometrium of the Uterus HUANG Li-xia;MA Li-guo 2016, 43 (2):  210-213.  Abstract ( 1067 )   PDF (771KB) ( 5793 )   Objective：To study the expression changes of interleukin-8 (IL-8) mRNA, matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) in eutopic endometrium of patients with endometriosis (EMs), and to confirm that it is involved in the occurrence and development of EMs. Methods：IL-8 mRNA, MMP-9 and VEGF protein expression in eutopic endometrium of EMs patients before and after treatment and the healthy control group were detected. Results：The expression of IL-8 mRNA in EMs group before treated was higher than that in control group, and the expression of IL-8 mRNA was decreased after treatment (both P＜0.05). Positive expression rate of MMP-9 and VEGF protein in endometriosis patients before treated were higher than that of the control group and the patients after treatment (P＜0.05). IL-8 mRNA and MMP-9 protein expression and VEGF protein expression existed positive correlation (rs=0.842, 0.851, all P＜0.05). Conclusions：Complete surgical excision of endometriosis lesions combined with drug therapy can improve the microenvironment of endometriosis and to delay its recurrence. IL-8, MMP-9 and VEGF had synergistic effect in endometriosis occurrence and development. Related Articles | Metrics

Select

Influence of Taishan Panshi San to Recurrent Spontaneous Abortion Mouse Maternal-fetal Interface Th1/Th2 Cytokines and Pregnancy Prognosis XU Chun-yan;SUN Jing 2016, 43 (2):  214-216.  Abstract ( 1013 )   PDF (773KB) ( 5845 )   Objective：To investigate the effects of Taishan Panshi San on the maternal-fetal interface Th1/Th2 cytokines and pregnancy outcomes of recurrent abortion mouse, to provide a new experimental basis for clinical application of Taishan Panshi San. Methods：Classic modeling approach DBA/2 mice CBA/J hybridization, get recurrent abortion mouse models, 60 CBA/J mice that randomly caged with DBA/2 mice were divided into model group pregnancy, low Chinese medicine dose group, middle Chinese medicine dose group, high Chinese medicine dose group and positive control group; 10 pregnant CBA/J mice mated with BALB/C as a normal pregnancy. 14 days after pregnancy pregnant rats were sacrificed to observe the mouse placenta′s missing, and to extract oocytes cultured fetal community, 24 hours latter cell supernatants were collected, Elisa assay supernatant TNF-α, IFN-γ, IL-4, IL-10 content. Results：Compare with model group, the recurrent miscarriage mouse placenta loss ratio was significantly improved after treated with Taishan Panshi San, cytokine Th1 and IFN-γ were significantly decreased in cell supernatants of mother-fetal bound, Th2 cytokines IL-4 and IL-10 were significantly increased, Th1/Th2 immunoregulatory imbalance improved significantly, the high dose Chinese tradition medicine group was the most obvious. Conclusions：Taishan Panshi San can significantly improve the placenta lost situation of recurrent abortion mouse, the specific mechanism may be achieved by adjusting the balance of Th1/Th2 immune regulation. Related Articles | Metrics

综述

Select

Impact of Maternal Exposure to Fine Particulate Matter on Pregnancy Outcomes LI Qiu-ping;SHAO Yong 2016, 43 (2):  217-220.  Abstract ( 1123 )   PDF (792KB) ( 5791 )   Recently, it has been showed in clinical, epidemiological and animal studies that maternal exposure to air fine particulate matter during pregnancy can resulted in stillbirth, abortion, fetal growth restriction, low birth weight, preterm birth and other adverse pregnancy outcomes, while the potential mechanisms is uncertain. As studies have proved, PM2.5 may cause DNA injury that leads to stillbirth, abortion and congenital abnormalities, it can also change the function of placenta to restrict fetal growth. Besides, PM2.5 exposure during pregnancy can also induce changes in the construction of placenta that may be a key point to preterm premature rupture of membrane and preterm birth. This review will provide a summary of the adverse pregnancy outcomes of PM2.5 exposure on the developing fetus. Related Articles | Metrics

Select

The Association of PM2.5 with Adverse Pregnancy Outcomes WU Si-yu;HOU Hai-yan;CHEN Juan;CHEN Ya-qiong;JIANG Jing 2016, 43 (2):  221-225.  Abstract ( 1226 )   PDF (840KB) ( 5792 )   Through the review of nearly 40 domestic and foreign literature, the formation, components and toxicity of PM2.5 and its association with adverse birth outcomes (ABOs) including low birth weight (LBW), preterm birth (PTB), stillbirth, gestational diabetes mellitus (GDM) and pre-eclampsia are investigated. PM2.5 mainly consists of element carbon, organic carbon, inorganic matter and heavy metals. Coal burning and traffic are the main sources of urban PM2.5. Heavy metals and polycyclic aromatic hydrocarbons were the main toxic components of PM2.5, which could exert adverse effect on pregnancy outcome through direct placental permeation, affecting maternal endocrine and immune system, inducing oxidative stress or changing genetic materials. At last, there is still mystery about the association of PM2.5 and GDM and pre-eclampsia. Nevertheless, PM2.5 is convincingly thought to be a risk factor of LBW, PTB and stillbirth. Related Articles | Metrics

Select

Long-term Consequences of Intrauterine Nutrition for Fetal Vascular Health WANG Yan -ping;CHEN Xu 2016, 43 (2):  226-229.  Abstract ( 1022 )   PDF (824KB) ( 5807 )   There are many instances in life when the environment plays a critical role in the health outcomes of an individual, especially those experienced in fetal and neonatal life. The most detrimental environmental problems encountered during this critical growth period are changes in nutrition to the growing fetus and newborn. Disturbances in the supply of nutrients and oxygen to the fetus can not only lead to adverse fetal growth patterns, but also be associated with the development of features of metabolic syndrome in adult life. Energy and/or protein restriction is the most critical determinant for fetal programming. There is a growing body of evidence that improper intrauterine nutrition may negatively influence vascular health in later life. It concerns both undernutrition and overnutrition. Imbalanced intrauterine nutrition seems to influence vascular health in the offspring by both an increase in their cardiometabolic risk factors and direct influence on vascular structure and function. Maternal malnutrition may result in intrauterine growth retardation and, in turn, metabolic disorders such as insulin resistance, diabetes, hypertension, and dyslipidemia, can also enhanced risk of atherosclerosis and cardiovascular death in the offspring. Related Articles | Metrics

论著

Select

Effect of Continuous Stretch on Contractile Protein Expression of Uterine Smooth Muscle under High Glucose Circumstance MAO Jing;HAN Zhi-min;ZHOU Mu-lan;DONG Ling-ling 2016, 43 (2):  230-233.  Abstract ( 1198 )   PDF (840KB) ( 5812 )   Objective：To analyze the effect of continuous stretch on oxytocin receptors and prostaglandin receptors expression of uterine smooth muscle cells under high glucose circumstance. Methods：Rat uterine smooth muscle cells were separated and cultured under high glucose circumstance for different durations, then we detected oxytocin receptors and prostaglandin receptors expression changes of smooth muscle cells. Continuous stretch was forced on cells under high glucose circumstance to determine synergistic effect of stretch and high glucose. At the same time, as a control team, advanced glycation end products (AGEs) inhibitor (sRAGE) was added to antagonize the effect of high glucose, then evaluate the expression of oxytocin receptors and prostaglandin receptors. Results：Both high glucose circumstance and continuous stretch could lead to oxytocin receptors and prostaglandin receptors expression of rat uterine smooth muscle cells increasing. Exerting high glucose and continuous stretch simultaneously could promote oxytocin receptors and prostaglandin receptors expressions even further and synergistic effect could be detected. After adding sRAGE, oxytocin receptors and prostaglandin receptors expressions decreased to some extend, but not completely, still higher than the normal glucose teams. Conclusions：Both continuous stretch and high glucose could promote oxytocin receptors and prostaglandin receptors expressions and there exist a synergistic effect between them. AGEs was involved in this process, but this is not the only signal pathway. Related Articles | Metrics

Select

The Effect of Compound Salvia Miltiorrhiza Injection on the Endothelial Function and Inflammation Level of Patients with Early Onset Severe Pre-eclampsia GUO Yong-ping;YANG Feng;WANG Xiao-jing 2016, 43 (2):  234-237.  Abstract ( 1317 )   PDF (820KB) ( 5842 )   Objective：To investigate the effect of compound salvia miltiorrhiza injection on early onset severe pre-eclampsia patients and their neonatals. Methods：Total 130 patients diagnosed with early onset severe pre-eclampsia between March 2013 and March 2015 in Baoji Maternal and Child Care Service Centre were randomly divided into compound salvia miltiorrhiza injection group (67 cases) and control group (63 cases). Control group patients gave spasmolysis, calm, step-down diuresis, appropriate and dexamethasone to promote fetal lung maturity, high-protein low-fat diet routine therapy, salvia miltiorrhiza group patients gave compound salvia miltiorrhiza injection on the basis of control group treatment, both groups of patients were treated with two weeks. Nitric oxide, endothelin-1(ET-1), TNF-α and IL-6 levels were detected and compared before and after treatment in two groups. And also, the blood pressure before and after the treatment, childbirth neonatal gestational age, postpartum neonatal deaths, quality of the placenta, survival neonatal weight and 1 min Apgar score were compared between two groups. Results：The indicators of the two groups of patients had no statistical differences before treatment (P＞0.05). ET-1, ET-1/NO, TNF-α, IL-6 levels, systolic pressure, diastolic pressure and mean arterial pressure were reduced by two treatment methods in early onset severe pre-eclampsia patients, nitric oxide was elevated significantly (P＜0.05). The degree of changes in ET-1, ET-1/NO, TNF-α, IL-6 and nitric oxide in compound salvia miltiorrhiza injection group before and after treatment were greater than control group (P＜0.001). Salvia miltiorrhiza treatment group neonatal mortality is lower than the control group, to those who gave live births, childbirth neonatal gestational age was longer in the salvia miltiorrhiza group (P＜0.001). Quality of the placenta, neonatal weight, quality of the placenta and 1 min Apgar score in survival subjects were improved in compound salvia miltiorrhiza injection group than control group (P＜0.001). Conclusions：Compound salvia miltiorrhiza injection improved prognosis of early onset severe pre-eclampsia patients by mechanism of anti-inflammation and regulation of endothelial function. Related Articles | Metrics

Select

The Treatment Experience of Peg-suture to Postpartum Hemorrhage Caused by Uterine Inertia in Cesarean Section HE Mei;ZHOU Wen-hong 2016, 43 (2):  238-240.  Abstract ( 1789 )   PDF (814KB) ( 6579 )   Objective：To investigate the clinical value of peg-suture to postpartum hemorrhage caused by uterine inertia. Methods：There are 218 cases of postpartum hemorrhage during Jan. 2007 to May. 2014, 82 cases of them received conservative treatment (uterine massotherapy, oxytocin, hemabate and so on), there is no effect. Observing group include 40 cases received peg-suture, control group 1 include 20 cases received B-Lynch suture, control group 2 include 22 cases received gauze pocking of uterine. The patients with complete uterine inertia were performed ovarian artery ligation and uterine artery ligation. To compare the effect of hemostasis, healing of uterine incision, blood flow of uterine, complication, uterine involution, ejection of lochia and reappearance of menstruation. Results：The hemostasis rate in each of the 3 groups are 100%, 85.00% and 81.8%, the differences are significant (P＜0.05). In the three groups, healing of uterine incision, blood flow of uterine and ovary are well. In the observing group, 6 cases run fever, in control group 1, 3 cases run fever, 1 case get intestinal obstruction, in control group 2, 6 cases run fever, 2 cases the gauze are sutured with incision of uterine, the differences are not significant (P＞0.05). In the three groups, the difference of uterine involution, ejection of lochin and reappearance of menstruation are not significant (P＞0.05). Conclusions：To postpartum hemorrhage caused by uterine inertia, peg-suture has high rate of success, good hemostasis effect and low complication, operation time is short, manipulation is simple, so is worth spreading. Related Articles | Metrics