Abstract: Objective: To detect the expression of micro RNA-210 (miR-210) and hypoxia-inducible factor 1α (HIF-1α) in placenta of pre-eclampsia (PE) patients, and analyze the relationship between them as well as the correlation between the expression and clinicopathological characteristics of PE patients. Methods: 46 pregnant women with PE admitted to our hospital from January 2016 to February 2018 were selected as the study subjects. According to the severity of PE, 21 cases were divided into mild group and 25 cases into severe group. Another 34 pregnant women with normal pregnancy examination and cesarean delivery were selected as the control group. The clinical symptoms of PE patients were analyzed by routine biochemical indicators test. Fluorescent quantitative PCR was used to detect the expression of miR-210 and HIF-1α mRNA in placenta. Immunohistochemical staining was used to detect the expression of HIF-1α in placenta. And the correlation between the expressions of miR-210 and HIF-1α in placenta of PE patients and their relationships with the basic pathological data of patients were analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of miR-210 and HIF-1α mRNA expression levels in PE. Results: Systolic blood pressure (SBP), diastolic blood pressure (DBP) and urinary protein (PRO) levels in PE patients were significantly higher than those in the control group (P＜0.05), and severe PE group were significantly higher than mild PE group (P＜0.05). The levels of miR-210 and HIF-1α in placenta of PE patients were significantly higher than those of control group (P＜0.05), and severe PE group were significantly higher than mild PE group (P＜0.05). The levels of miR-210 and HIF-1α in placenta were correlated with the levels of SBP, DBP, PRO and the severity of PE (P＜0.05). The expressions of miR-210 and HIF-1α in placenta of PE patients were positively correlated (P＜0.05). ROC analysis showed that the cut-off values of miR-210 and HIF-1α mRNA were 1.06 and 1.27, and the sensitivity of PE diagnosis was 80.43% and 78.26%, the specificity was 70.59% and 82.35%. Conclusions: MiR-210 and HIF-1α are highly expressed in the placenta of PE patients, and they are positively correlated with each other, and participate in the occurrence and development of PE disease.

Key words: Pre-eclampsia, MicroRNAs, Hypoxia-inducible factor 1, alpha subunit, Placenta