Abstract: Objective：To observe the expression of P16 protein in cervix tissue by immunohistochemical method and 10% neutral buffer Faure Marin (traditional stationary liquid), and explore the feasibility of replacing it with 10% neutral buffer Faure Marin. Methods：245 specimens of cervical tissue were collected from the outpatient department and the department of gynaecologic inpatient department of Zhongshan BOAI Hospital Affiliated to Southern Medical University from March 2015 to November 2017. There were 52 cases of normal and chronic cervicitis, 87 cases in group LSIL, 61 in group HSIL and 45 in cervical cancer group. 2 pieces of the same lesion were collected and randomly divided into two groups, which were named group A and B. In group A, 245 sections were made by 10% neutral buffered formalin, and 245 sections were made by polyhydroxy acrylic acid in group B. Immunohistochemical method was used to detect the expression of P16 protein in cervical tissue. Results：①Under the biological microscope, the two groups of A and B slices had clear background, positive collocation, accurate location, strong positive intensity, bright and fresh color, and no specific staining, negative and positive staining results were reliable. ②In group A, the positive rate of p16 protein in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group were 5.77%, 35.63%, 80.33% and 100.00%, respectively. The positive rate of p16 protein in B group was 5.77%, 33.33%, 81.97% and 100.00% respectively in chronic cervicitis group, LSIL group, HSIL group and cervical cancer group. There was no significant difference in the positive rate of P16 protein expression between A and B groups (P＞0.05). Conclusions：Using polyhydroxyacrylic acid instead of 10% neutral buffer Faure Marin has no significant difference on the positive rate of P16 protein expression in cervical tissue by immunohistochemical method. Polyhydroxyacrylic acid as a stationary liquid has certain value in the detection of P16 protein expression in cervical tissue by immunohistochemical method.

Key words: Polyhydroxyl acrylic acid, Formalin, Immunohistochemistry , Genes, p16