Abstract: Objective：To explore the clinical significance and management strategy with atypical squamous cells of undetermined significance. Methods：A total of 216 cases involved in this study were out-patients who were diagnosed ASCUS by LCT test and further biopsied under the colposcope from January，2008 to December，2009 in Tianjin Medical University General Hospital. 140 cases of them were combined by HPV-DNA test. Results：①The incidence rate of CIN，high-grade CIN and cervical cancer was 44.44% and 20.37%. ②The average age of high-grade CIN and cervical cancer was（44.57±11.23）years old，which was older than wart（31.56±9.69）years old and low-grade CIN（39.48±12.20）years old（P＜0.05）. ③The highest probility of hr-HPV subtype in ASCUS with high-grade CIN and cervical cancer was HPV16 with percentage of 41.67%. HPV58，18，52 are other common subtypes. ④The relevant ratio of CIN in ASCUS patient with positive HPV-DNA test is 55.56%，which was higher than both immediate colposcope without HPV-DNA test with the ratio of 38.16%（χ2=5.41，P＜0.05）and negative HPV-DNA test with the ratio of 31.25%（χ2=5.83，P＜0.05）. ⑤The Negative Predictive Value of HSIL of HPV test was 93.75%. Conclusions：The pathologic type of ASCUS was complicated. Warts were more xommon in patients younger than 32ys. Patients older than 40ys especially combined with HPV16， 58， 18， 52 infection were supposed to be noticed that whether there were high-grade cervical lesions and cervical cancer. ASCUS patients should take HPV test firstly and positive patients taking colposcope could increase the relevance ratio of CIN. Repeated LCT was feasible for negative HPV patients.

Key words: Uterine cervical diseases, Cervix uteri, Colposcopy, Biopsy, Cytodiagnosis